Figure 5 from Inhibition of Extracellular Matrix Protein Fibulin-3 Reduces Immunosuppressive Signaling and Increases Macrophage Activation in Glioblastoma

Anti–fibulin-3 antibody increases intratumoral presence of proinflammatory TAMs. A, Representative images of intracranial GSC-derived tumors (GBM09) treated with humanized anti–fibulin-3 mAb428.2 or its IgG control. Tissues were stained to reveal TAM density (IBA1) and expression of CD206 in TAMs. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 200 μm. B and C, Quantification of total TAMs (B) and CD206 expression in TAMs (C) in tumors treated with mAb428.2 or its control; data were analyzed by Welch’s corrected t test. D, Expression of CSF-1, TGF-β1, and CD47 in three different GSC cultures treated with mAb428.2 or its isotype control. Analysis was done by multiple paired t tests for each GSC line.