Figure 2 from Inhibition of Extracellular Matrix Protein Fibulin-3 Reduces Immunosuppressive Signaling and Increases Macrophage Activation in Glioblastoma

Fibulin-3 expression correlates with an immunosuppressive signature in GBM. A, Correlation of fibulin-3 mRNA expression with the expression of CSF-1, TGF-β1, and the checkpoints CD47 and CD274 (PD-L1), extracted from The Cancer Genome Atlas GBM dataset. B, Correlation of fibulin-3 with the markers indicated in (A) in GBM tumors separated by their bulk transcriptional subtype. C, Heatmap showing top gene expression changes in two GSC lines (GBM34 and GBM09) stably transfected with control or fibulin-3 short hairpin RNAs. D, Changes in gene expression observed in hallmark signatures for inflammatory response and signaling by IFN-γ and -α (data analyzed using GSEA v.4.3.3), comparing fibulin-3–deficient GSCs (fib-3 KD) vs. control cells. KD, knockdown; NES, normalized enrichment score.