What type of study is this?

This is a In Vitro Study study (also classified as: Experimental Study, Quantitative Study).

September 16, 2025Journal of Inflammation ResearchOpen Access

Urolithin-C Suppresses Inflammation by Blocking NF-κB Signaling Pathway in LPS-Induced RAW 264.7 Macrophages

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Authors

VMVani Karadi Manjappa MVManjula M. Venkatappa DUDeepadarshan Urs

Discussion

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Overview

In vitro analysis reveals urolithin-C reduces pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophages, highlighting its therapeutic potential.

Key Points

Urolithin-C significantly suppresses inflammation by blocking the NF-κB signaling pathway in macrophages, and reduces pro-inflammatory cytokines such as IL-2, IL-6, and TNF-alpha.
At a concentration of 25 µg/mL, urolithin-C shows anti-inflammatory effects without cytotoxicity in RAW 264.7 macrophages, contrasting with higher doses that are toxic.
The study employed membrane stabilization, protease inhibition, and ELISA to quantify cytokines, showcasing urolithin-C as more potent than the positive control drug diclofenac.
Urolithin-C's mechanism involves downregulating pro-inflammatory gene expression while enhancing anti-inflammatory cytokine production, demonstrating its potential as an anti-inflammatory agent.

Cite This Study

Manjappa et al. (2025) studied this question.

synapsesocial.com/papers/68d46ccf31b076d99fa68f65 https://doi.org/10.2147/jir.s539273

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September 16, 2025Journal of Inflammation ResearchOpen Access

Urolithin-C Suppresses Inflammation by Blocking NF-κB Signaling Pathway in LPS-Induced RAW 264.7 Macrophages

View Full Paper

Ask AI

Bookmark

Authors

VMVani Karadi Manjappa MVManjula M. Venkatappa DUDeepadarshan Urs

Discussion

Member takes

Overview

In vitro analysis reveals urolithin-C reduces pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophages, highlighting its therapeutic potential.

Key Points

Urolithin-C significantly suppresses inflammation by blocking the NF-κB signaling pathway in macrophages, and reduces pro-inflammatory cytokines such as IL-2, IL-6, and TNF-alpha.
At a concentration of 25 µg/mL, urolithin-C shows anti-inflammatory effects without cytotoxicity in RAW 264.7 macrophages, contrasting with higher doses that are toxic.
The study employed membrane stabilization, protease inhibition, and ELISA to quantify cytokines, showcasing urolithin-C as more potent than the positive control drug diclofenac.
Urolithin-C's mechanism involves downregulating pro-inflammatory gene expression while enhancing anti-inflammatory cytokine production, demonstrating its potential as an anti-inflammatory agent.

Cite This Study

Manjappa et al. (2025) studied this question.

synapsesocial.com/papers/68d46ccf31b076d99fa68f65 https://doi.org/10.2147/jir.s539273

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