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The research aims to characterize the bile salt hydrolase activity and genomic features of L. brevis M3R3 for its probiotic potential.

Bile Salt Hydrolase Activity in the Food-Derived Strain Levilactobacillus brevis M3R3: Genomic and Functional Characterization

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The research aims to characterize the bile salt hydrolase activity and genomic features of L. brevis M3R3 for its probiotic potential.
Conducted kinetic growth assays to evaluate BSH activity
Performed bile salts deconjugation analysis using UHPLC/HR-MS
Executed whole-genome sequencing for genomic characterization
Conducted safety assessments for antimicrobial resistance and virulence genes
Conducted phylogenetic analysis of BSH proteins
Strain M3R3 showed growth inhibition with different conjugated bile salts
Complete deconjugation of glyco-conjugated bile salts confirmed via UHPLC/HR-MS
Phylogenetic analysis identified M3R3's bsh genes in distinct protein clusters
No antimicrobial resistance or virulence genes detected in the genome
Strain maintained growth under mixed bile salt exposure despite reduced biomass

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Genomic and functional characterizations show L. brevis M3R3 maintains growth under bile salt exposure, indicating probiotic potential.

Agolino et al. (2025) studied this question.

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