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November 18, 2025The Journal of Infectious Diseases

Detection of Carbapenem-Resistant Bacteria in Skilled Nursing Facility Wastewater

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Authors

SLSusanna LenzSBSubhasish BiswasITIvy Terry

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Overview

Wastewater surveillance reveals antimicrobial resistance in healthcare facilities, highlighting CR bacteria presence.

Key Points

  • To investigate the presence of carbapenem-resistant bacteria in skilled nursing facility wastewater.
  • Collected wastewater samples from three skilled nursing facilities weekly for six months.
  • Used selective chromogenic media and culture methods to identify carbapenem-resistant bacteria.
  • Applied PCR to screen for common carbapenemase genes within isolates and pooled samples.
  • Utilized whole genome sequencing for comprehensive analysis of antimicrobial resistance genes.
  • Identified 209 bacterial isolates across over 20 genera using MALDI-TOF.
  • Detected clinically relevant resistant bacteria including Acinetobacter baumannii and Enterobacter cloacae.
  • Confirmed blaKPC presence in Facilities A and C, with no PCR positives in Facility B.
  • Achieved 100% concordance between WGS and PCR results for targeted genes.

Cite This Study

Lenz et al. (2025) studied this question.

synapsesocial.com/papers/6924fef9c0ce034ddc351ed4https://doi.org/10.1093/infdis/jiaf534
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Detection of Carbapenem-Resistant Bacteria in Skilled Nursing Facility Wastewater2025
  2. 2Evaluation of Detection Methods for Wastewater Surveillance of Antimicrobial-Resistant Bacteria from Healthcare Facilities2026
  3. 3Could the load of carbapenemase genes in hospital wastewater be a proxy for the epidemiology of emerging resistance to carbapenems in humans?2025
  4. 4Establishing a Practical Approach to Sewer Monitoring for Antimicrobial Resistance Genes and Organisms at Healthcare Facilities2025
  5. 5Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria2024 · 2 citations