What is the clinical evidence from this study?

Study design: Other. Population: Mitral valve disease and hypertrophic obstructive cardiomyopathy (n=12). Intervention: Verapamil enantiomers ((-)-verapamil and (+)-verapamil) vs. Baseline (no verapamil). Primary outcome: Cardiodepression and [3H]-nimodipine binding inhibition.

April 1, 1985British Journal of PharmacologyOpen Access

Relationship between the stereoselective negative inotropic effects of verapamil enantiomers and their binding to putative calcium channels in human heart

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Key result

Both (-)-verapamil and (+)-verapamil caused cardiodepression and inverted the positive force-frequency staircase in human ventricular tissue while allosterically inhibiting calcium channel binding.

Population

Ventricular preparations and membrane particles from patients with mitral disease and hypertrophic…

Design

Preclinical

Authors

DFD. FerryEli Lilly (United States)HGHartmut GlossmannJohannes Kepler University of Linz AKAlberto J. KaumannElectrophysiology

Discussion

Member takes

Implication

Ex vivo human tissue effects warrant no change in verapamil use; leaves open enantiomer-specific actions in patients.

Structured PICO

Population

Ventricular preparations and membrane particles from patients with mitral disease and hypertrophic obstructive cardiomyopathy (HOCM), including subendocardial strips from 5 patients with HOCM.

Intervention

(-)-verapamil and (+)-verapamil

Outcome

Cardiodepression (negative inotropic effect) and inhibition of [3H]-nimodipine binding to calcium channelssurrogate

The similar potency ratios for binding and cardiodepression suggest a common locus of action for verapamil enantiomers within the calcium channel.

Limitations

Limited human tissue available per patient restricted the number of assay points
In vitro study design may not fully reflect in vivo conditions

Cite This Study

Ferry et al. (1985) studied Mitral valve disease and hypertrophic obstructive cardiomyopathy (n=12). Verapamil enantiomers ((-)-verapamil and (+)-verapamil) vs. Baseline (no verapamil) was evaluated on Cardiodepression and [3H]-nimodipine binding inhibition. Both (-)-verapamil and (+)-verapamil caused cardiodepression and inverted the positive force-frequency staircase in human ventricular tissue while allosterically inhibiting calcium channel binding.

synapsesocial.com/papers/6a08db7e73760a4edcd6048e https://doi.org/10.1111/j.1476-5381.1985.tb17375.x

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April 1, 1985British Journal of PharmacologyOpen Access

Relationship between the stereoselective negative inotropic effects of verapamil enantiomers and their binding to putative calcium channels in human heart

View Full Paper

Ask AI

Bookmark

Key result

Both (-)-verapamil and (+)-verapamil caused cardiodepression and inverted the positive force-frequency staircase in human ventricular tissue while allosterically inhibiting calcium channel binding.

Population

Ventricular preparations and membrane particles from patients with mitral disease and hypertrophic…

Design

Preclinical

Authors

DFD. FerryEli Lilly (United States)HGHartmut GlossmannJohannes Kepler University of Linz AKAlberto J. KaumannElectrophysiology

Discussion

Member takes

Implication

Ex vivo human tissue effects warrant no change in verapamil use; leaves open enantiomer-specific actions in patients.

Structured PICO

Population

Ventricular preparations and membrane particles from patients with mitral disease and hypertrophic obstructive cardiomyopathy (HOCM), including subendocardial strips from 5 patients with HOCM.

Intervention

(-)-verapamil and (+)-verapamil

Outcome

Cardiodepression (negative inotropic effect) and inhibition of [3H]-nimodipine binding to calcium channelssurrogate

The similar potency ratios for binding and cardiodepression suggest a common locus of action for verapamil enantiomers within the calcium channel.

Limitations

Limited human tissue available per patient restricted the number of assay points
In vitro study design may not fully reflect in vivo conditions

Cite This Study

synapsesocial.com/papers/6a08db7e73760a4edcd6048e https://doi.org/10.1111/j.1476-5381.1985.tb17375.x

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

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