What does this research mean for the field?

Elevation of intracellular cAMP and cGMP independently inhibits vascular smooth-muscle cell proliferation, but this anti-proliferative effect is not mediated by the inhibition of calcium mobilization. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To investigate how cyclic AMP and GMP affect cellular proliferation and Ca2+ mobilization in rabbit aortic smooth-muscle cells.

December 1, 1992Biochemical JournalOpen Access

Inhibition of proliferation, but not of Ca2+ mobilization, by cyclic AMP and GMP in rabbit aortic smooth-muscle cells

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To investigate how cyclic AMP and GMP affect cellular proliferation and Ca2+ mobilization in rabbit aortic smooth-muscle cells.
Compared effects of cyclic nucleotide analogues and agents elevating cyclic nucleotides in rabbit aortic smooth-muscle cells.
Used 1 mM concentrations of 8-bromo cAMP and 8-bromo cGMP to evaluate effects on proliferation.
Measured intracellular levels of cAMP, cGMP, and Ca2+ in the presence of other agents like isobutylmethylxanthine and forskolin.
1 mM-8-bromo cAMP inhibited proliferation by 78%, while 1 mM-8-bromo cGMP inhibited it by 42%.
Forskolin increased cAMP by 5-fold and inhibited proliferation by 87%.
SIN-1 increased cGMP by 4-fold and inhibited proliferation by 48%, with no effect on Ca2+ mobilization.

Structured PICO

Population

First-passage rabbit aortic vascular smooth-muscle cells

Intervention

Analogues of cyclic AMP (cAMP) and cyclic GMP (cGMP) (e.g., 1 mM-8-bromo cAMP, 1 mM-8-bromo cGMP) and agents that elevate intracellular concentrations of cyclic nucleotides (forskolin, SIN-1, isobutylmethylxanthine)

Comparator

Control/baseline conditions

Outcome

Cellular proliferation and intracellular free Ca2+ concentration (Ca2+ mobilization)surrogate

Elevation of intracellular cAMP and cGMP independently inhibits vascular smooth-muscle cell proliferation without affecting Ca2+ mobilization.

Discussion

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Implication

These pathways warrant further investigation as antiproliferative targets; leaves open clinical translation from animal cell data.

Cite This Study

Assender et al. (1992) studied this question.

synapsesocial.com/papers/6a12dccdd61942a939c080d0 https://doi.org/10.1042/bj2880527

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Inhibition of proliferation, but not of Ca2+ mobilization, by cyclic AMP and GMP in rabbit aortic smooth-muscle cells

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Inhibition of proliferation, but not of Ca2+ mobilization, by cyclic AMP and GMP in rabbit aortic smooth-muscle cells

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider