Key result
Physiological shear stress increased PDGF A mRNA levels by more than 10-fold and PDGF B mRNA levels by 2- to 3-fold in cultured human umbilical vein endothelial cells (P<0.05).
Why the study?
Does shear stress increase PDGF A and B mRNA levels in cultured human umbilical vein endothelial cells?
Population
Cultured human umbilical vein endothelial cells (hUVEC)
Comparison
Shear stress vs Control values (baseline/no shear stress)
Design
Preclinical
Follow-up
Up to 4 hours
Authors
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Does not alter practice; hypothesis-generating for shear-mediated PDGF regulation in vascular adaptation.
Does shear stress increase PDGF A and B mRNA levels in cultured human umbilical vein endothelial cells?
Effect estimate: >10-fold increase (PDGF A), 2- to 3-fold increase (PDGF B)
p-value: p=<0.05
Shear stress increases PDGF A and B mRNA levels in endothelial cells, suggesting a mechanism for flow-mediated vascular adaptation.
Hsieh et al. (1991) studied this question. Shear stress vs. Control was evaluated on PDGF A and B chain mRNA levels (>10-fold increase (PDGF A), 2- to 3-fold increase (PDGF B), p=<0.05). Physiological shear stress increased PDGF A mRNA levels by more than 10-fold and PDGF B mRNA levels by 2- to 3-fold in cultured human umbilical vein endothelial cells (P<0.05).
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