Key result
PCR using new inosine-containing group II primers achieved a higher detection rate for small round-structured viruses in faecal samples (68%) compared to immune electron microscopy (60%).
Why the study?
Does PCR using inosine-containing primers improve the detection rate of SRSVs in faecal samples compared to immune EM?
Population
Patients involved in two outbreaks of gastroenteritis at Lymington and Southampton hospitals in the UK
Comparison
Polymerase chain reaction using new… vs Immune electron microscopy and conventional…
Design
Other
Authors
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May aid SRSV diagnosis in clinical samples; leaves open validation for routine use and outbreak tracking.
Observational
Yes
Does PCR using inosine-containing primers improve the detection rate of SRSVs in faecal samples compared to immune EM?
Absolute Event Rate: 68% vs 60%
PCR with inosine-containing primers improves the detection of SRSVs in clinical samples and enables molecular epidemiological tracking of outbreaks.
Green et al. (1995) conducted an observational in Gastroenteritis (small round-structured virus infection). Polymerase chain reaction (PCR) using new inosine-containing primers vs. Immune electron microscopy (EM) was evaluated on Detection rate of SRSVs in faecal samples. PCR using new inosine-containing group II primers achieved a higher detection rate for small round-structured viruses in faecal samples (68%) compared to immune electron microscopy (60%).
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