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April 1, 1993Circulation Research

Flow-related responses of intracellular inositol phosphate levels in cultured aortic endothelial cells.

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Population

Cultured bovine aortic endothelial cells (BAECs) seeded and grown to confluence on large polyester sheets

Comparison

High shear stress at 30 dynes/cm2 or 60… vs Low shear stress (LS) at < 0.5 dyne/cm2

Design

Preclinical

Follow-up

24 hours

Authors

APA. R. PrasadThe University of Texas Health Science Center at San AntonioSLStephen A. LoganPlastic Electronic (Austria)RNRobert M. NeremVascular Medicine

Discussion

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Implication

IP3 may mediate endothelial adaptation to shear; extends in vitro mechanotransduction data but leaves open in vivo relevance.

Structured PICO

P
Population
Cultured bovine aortic endothelial cells (BAECs) seeded and grown to confluence on large polyester sheets
I
Intervention
High shear stress (HS) at 30 dynes/cm2 or 60 dynes/cm2 for periods ranging from 15 seconds to 24 hours, with or without 5 mM neomycin pretreatment
C
Comparator
Low shear stress (LS) at < 0.5 dyne/cm2
O
Outcome
Intracellular release of inositol phosphates (IP3) measured at various time points up to 24 hourssurrogate

Elevated wall shear stress in cultured endothelial cells triggers an early biphasic increase in inositol trisphosphate, which mediates subsequent cell shape change and orientation.

Cite This Study

Prasad et al. (1993) studied this question.

synapsesocial.com/papers/6a1ab4bb9fa30811a0b902c8https://doi.org/10.1161/01.res.72.4.827
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Dynamic Response of Vascular Endothelial Cells to Fluid Shear Stress1981 · 1,144 citations
  2. 2Fluid shear stress modulates cytosolic free calcium in vascular endothelial cells1992 · 349 citations
  3. 3Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides1983 · 1,967 citations
  4. 4Flow patterns and spatial distribution of atherosclerotic lesions in human coronary arteries.1990 · 857 citations
  5. 5Phorbol ester and neomycin dissociate bradykinin receptor-mediated arachidonic acid release and polyphosphoinositide hydrolysis in Madin-Darby canine kidney cells. Evidence that bradykinin mediates noninterdependent activation of phospholipases A2 and C.1988 · 169 citations