What does this research mean for the field?

In human adipocytes, the genes for renin, angiotensin-converting enzyme, and the angiotensin II type 1 receptor are upregulated in obese hypertensive individuals, while angiotensinogen is downregulated, and hydrocortisone specifically increases angiotensin II type 1 receptor expression. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To investigate the expression of renin-angiotensin system genes in adipose tissue of obese hypertensive individuals and evaluate their hormonal regulation.

May 1, 2002Journal of Hypertension

Hormonal regulation of the human adipose-tissue renin–angiotensin system: relationship to obesity and hypertension

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Why the study?

How are renin-angiotensin system genes differentially expressed in adipose tissue of obese hypertensive subjects, and how are they hormonally regulated?

Population

30 women for the cross-sectional clinical study, and primary human mammary adipocytes from healthy women for…

Comparison

In vitro exposure to hydrocortisone, insulin… vs Lean normotensive vs obese normotensive vs obese…

Design

Cross-sectional

Authors

KGKerstin GorzelniakUnfallkrankenhaus Berlin SEStefan EngeliUniversitätsmedizin Greifswald JJJürgen JankeMax Delbrück Center

Discussion

Member takes

Implication

Adipose RAS upregulation may link obesity to hypertension; hypothesis-generating and should not yet change practice.

Key Points

To investigate the expression of renin-angiotensin system genes in adipose tissue of obese hypertensive individuals and evaluate their hormonal regulation.
Conducted a cross-sectional study analyzing subcutaneous abdominal adipocytes from 30 women (12 lean normotensive, 8 obese normotensive, and 10 obese hypertensive) characterized by anthropometry and 24-hour ambulatory blood pressure monitoring.
Treated primary cultured human mammary adipocytes with hydrocortisone, insulin, thyroxin, estradiol, and angiotensin II, measuring renin-angiotensin system gene expression via quantitative RT-PCR.
Angiotensinogen gene expression was significantly lower in both obese groups, whereas renin, angiotensin-converting enzyme, and angiotensin II type 1 receptor genes were significantly upregulated in obese hypertensive subjects.
Hydrocortisone significantly increased angiotensin II type 1 receptor mRNA and protein expression in a time- and dose-dependent manner, whereas other tested hormones had no significant effect.

Structured PICO

How are renin-angiotensin system genes differentially expressed in adipose tissue of obese hypertensive subjects, and how are they hormonally regulated?

Population

30 women (12 lean normotensive, 8 obese normotensive, 10 obese hypertensive) for the cross-sectional clinical study, and primary human mammary adipocytes from healthy women for the in vitro study.

Intervention

In vitro exposure to hydrocortisone, insulin, thyroxin, estradiol, and angiotensin II

Comparator

Lean normotensive vs obese normotensive vs obese hypertensive women (clinical); untreated/control adipocytes (in vitro)

Outcome

Expression of renin-angiotensin system genes in subcutaneous abdominal adipocytessurrogate

Renin-angiotensin system genes are differentially regulated in human obesity and hypertension, with upregulation of renin, ACE, and AT1 receptor genes in obese hypertensives.

Cite This Study

Gorzelniak et al. (2002) studied this question.

synapsesocial.com/papers/6a1bcf23ea84844e355f00ee https://doi.org/10.1097/00004872-200205000-00032

Topics

Hypertension management

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Hormonal regulation of the human adipose-tissue renin–angiotensin system: relationship to obesity and hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Hormonal regulation of the human adipose-tissue renin–angiotensin system: relationship to obesity and hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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