The study identifies multiple mRNA isoforms of human hnRNP protein A1, suggesting it is encoded by a multigene family.
Answers are grounded in this paper and cite their sources.
May prompt isoform-specific splicing studies; leaves open multigene family roles in cardiovascular RNA biology.
Protein A1 is one of the major component of mammalian ribonucleoprotein particles (hnRNP). Human protein A1 cDNA cloning and sequencing revealed the existence of at least two protein isoforms. Among the cDNAs examined, sequence differences were found both in the structural portion, leading to aminoacid changes (Tyr to Phe or Arg to Lys) and in the non translated 3'-region where two T-stretches of different length were observed. Interestingly one of the aminoacid substitutions falls into a consensus sequence common to many RNA binding proteins. Northern blot analysis of poly A+ RNAs from five human tissues revealed two mRNA forms of 1500 and 1900 n due to alternative polyadenylation. Analysis of genomic DNA showed at least 30 A1-specific sequences, some of which correspond to processed pseudogenes. These results suggest that protein A1 is encoded by a multigene family.
No takes yet. Share an insight, caveat, or question.
Buvoli et al. (1988) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: