Key result
In T7-BHK, HEK 293T, and HeLa cells, the translational efficiency of BVDV IRES was two to three times higher than that of EMCV IRES, indicating its potential as a replacement.
BVDV IRES demonstrates higher translational efficiency than the commonly used EMCV IRES in several mammalian cell lines, suggesting its potential as a superior alternative for T7 polymerase-driven cytoplasmic expression.
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May enhance vector efficiency in mammalian cells; leaves open EMCV replacement pending in vivo validation.
Ghassemi et al. (2017) studied this question. BVDV IRES vs. EMCV IRES was evaluated on Translational efficiency. In T7-BHK, HEK 293T, and HeLa cells, the translational efficiency of BVDV IRES was two to three times higher than that of EMCV IRES, indicating its potential as a replacement.
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