What question did this study set out to answer?

The aim is to develop a reliable radioimmunoassay for measuring brain natriuretic peptide levels in human plasma.

January 1, 1997Scandinavian Journal of Clinical and Laboratory Investigation

A new, fast and reliable radioimmunoassay of brain natriuretic peptide in human plasma. Reference values in healthy subjects and in patients with different diseases

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Why the study?

What are the reference values of plasma BNP in healthy subjects and patients with various diseases using a new radioimmunoassay?

Population

189 individuals, including 124 healthy subjects, 8 patients with congestive heart failure, 14 with chronic…

Design

Cross-sectional

Authors

KJKimmo JensenAalborg University Hospital JCJan CarstensOdense University Hospital PIPer IvarsenAarhus University Hospital

Discussion

Member takes

Implication

Establishes reference BNP values with a new assay; leaves open its diagnostic utility pending prospective validation.

Key Points

The aim is to develop a reliable radioimmunoassay for measuring brain natriuretic peptide levels in human plasma.
Developed a radioimmunoassay using antibodies raised in rabbits.
Utilized the iodogen method for tracer preparation and polyethylene glycol for separation of free and bound tracer.
Extracted BNP from plasma using Sep-Pak C18 cartridges.
In healthy subjects (N=124), the mean BNP level was 1.8 pmol l-1 (SD 1.0).
Median BNP levels in patient groups: 30.5 pmol l-1 in congestive heart failure (N=8); 50.5 pmol l-1 in chronic renal failure before dialysis (N=14); 7.8 pmol l-1 in liver cirrhosis (N=25); 5.0 pmol l-1 in essential hypertension (N=18).
Recovery of unlabelled BNP from plasma was 77.5 +/- 6.2%, with a detection limit of 0.55 pmol l-1.

Structured PICO

What are the reference values of plasma BNP in healthy subjects and patients with various diseases using a new radioimmunoassay?

Population

189 individuals, including 124 healthy subjects, 8 patients with congestive heart failure, 14 with chronic renal failure, 25 with liver cirrhosis, and 18 with essential hypertension.

Intervention

Measurement of plasma brain natriuretic peptide (BNP) using a newly developed radioimmunoassay.

Outcome

Plasma BNP levelssurrogate

A newly developed radioimmunoassay reliably measures plasma BNP, demonstrating elevated levels in patients with congestive heart failure and chronic renal failure compared to healthy subjects.

Cite This Study

Jensen et al. (1997) studied this question.

synapsesocial.com/papers/6a218d68582b7ad9ebabccda https://doi.org/10.3109/00365519709084604

Topics

Hypertension management HFrEF treatment Heart failure

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A new, fast and reliable radioimmunoassay of brain natriuretic peptide in human plasma. Reference values in healthy subjects and in patients with different diseases

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

A new, fast and reliable radioimmunoassay of brain natriuretic peptide in human plasma. Reference values in healthy subjects and in patients with different diseases

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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