August 16, 2006European Journal of EndocrinologyOpen Access

Adiponutrin gene is regulated by insulin and glucose in human adipose tissue

Q: What is the clinical evidence from this study?

Study design: Other. Population: Insulin resistance and Diabetes. Intervention: Insulin and glucose infusion (clamp studies) vs. Baseline / healthy subjects. Primary outcome: Adiponutrin gene expression (8.4-fold increase, p=0.008).

View Full Paper

Ask AI

Bookmark

Key result

Insulin and glucose infusion significantly induced adiponutrin gene expression in human adipose tissue, with an additive tenfold effect when combined (P=0.008).

Why the study?

Does insulin and/or glucose infusion regulate adiponutrin gene expression in human adipose tissue?

Population

Healthy lean subjects, type-1 diabetic patients with chronic hyperglycemia, and type-2 diabetics

Comparison

Euglycemic hyperinsulinemic clamp, hyperglycemic… vs Baseline levels or healthy subjects

Design

Other

Authors

MMMarthe MoldesInserm GBGeneviève BeauregardUniversität Hamburg MFMay FarajUniversité de Montréal

Discussion

Member takes

Implication

Adiponutrin may mediate nutrient effects on adipose tissue in diabetes; leaves open mechanistic and therapeutic roles.

Key Points

The aim is to understand how insulin and glucose regulate adiponutrin gene expression in human adipose tissue and its potential dysregulation in metabolic disorders.
Quantified adiponutrin gene expression using reverse transcriptase-quantitative PCR in subcutaneous adipose tissue.
Conducted euglycemic hyperinsulinemic clamp (EGHI), hyperglycemic euinsulinemic clamp, and hyperglycemic hyperinsulinemic (HGHI) clamp studies.
Analyzed adiponutrin gene expression in patients with varying degrees of insulin resistance.
Insulin infusion during EGHI increased adiponutrin expression by 8.4-fold (P = 0.008).
Glucose infusion led to a 2.2-fold increase in adiponutrin expression (P = 0.03).
Combined insulin and glucose infusion via HGHI resulted in a tenfold increase in adiponutrin expression (P = 0.008).

Structured PICO

Does insulin and/or glucose infusion regulate adiponutrin gene expression in human adipose tissue?

Population

Healthy lean subjects, type-1 diabetic patients, and type-2 diabetic patients evaluated for adiponutrin gene expression regulation by insulin and glucose.

Intervention

Euglycemic hyperinsulinemic clamp (EGHI), hyperglycemic euinsulinemic clamp, and hyperglycemic hyperinsulinemic (HGHI) clamp

Comparator

Baseline levels or healthy subjects

Outcome

Adiponutrin gene expression in subcutaneous adipose tissuesurrogate

Main Result

Effect estimate: 8.4-fold increase

p-value: p=0.008

Adiponutrin gene expression in human adipose tissue is strongly regulated by insulin and glucose, and is dysregulated in type 1 and type 2 diabetes.

Cite This Study

Moldes et al. (2006) studied Insulin resistance and Diabetes. Insulin and glucose infusion (clamp studies) vs. Baseline / healthy subjects was evaluated on Adiponutrin gene expression (8.4-fold increase, p=0.008). Insulin and glucose infusion significantly induced adiponutrin gene expression in human adipose tissue, with an additive tenfold effect when combined (P=0.008).

synapsesocial.com/papers/6a2241bb00d082f62f9723c4 https://doi.org/10.1530/eje.1.02229

View Full Paper

Ask AI

Bookmark

Adiponutrin gene is regulated by insulin and glucose in human adipose tissue

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Adiponutrin gene is regulated by insulin and glucose in human adipose tissue

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider