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November 1, 1992Journal of VirologyOpen Access

Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells

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Key result

Poliovirus 2A acts as a trans-acting protein while 2B and potentially other viral proteins are cis-acting during viral RNA replication in vivo.

Population

HeLa cells transfected with full-length and subgenomic poliovirus RNAs

Comparison

Transfection with poliovirus RNAs containing… vs Full-length viral RNA transcripts or…

Design

Preclinical

Authors

PCPhil CollisUniversity of BirminghamBOBrian J. O'DonnellUniversity of FloridaDBDavid J. BartonUniversity of Colorado Anschutz Medical Campus

Discussion

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Implication

Does not alter polio management; leaves open targeted antiviral strategies pending further validation.

Structured PICO

P
Population
HeLa cells transfected with full-length and subgenomic poliovirus RNAs
I
Intervention
Transfection with poliovirus RNAs containing deletion mutations (in-frame and out-of-frame deletions in P1, P1-2A, P1-2A-2B)
C
Comparator
Full-length viral RNA transcripts or cotransfection with helper RNA
O
Outcome
Viral RNA replication efficiency and genetic complementationsurrogate

The study demonstrates that poliovirus protein 2A acts in trans, while 2B acts in cis during viral RNA replication, supporting a model linking translation of plus-strand RNA with replication complex formation.

Cite This Study

Collis et al. (1992) studied Poliovirus RNA replication. Transfection of full-length and subgenomic poliovirus RNAs was evaluated on Viral RNA replication and genetic complementation. Poliovirus 2A acts as a trans-acting protein while 2B and potentially other viral proteins are cis-acting during viral RNA replication in vivo.

synapsesocial.com/papers/6a225cce7d05348b2ab600cahttps://doi.org/10.1128/jvi.66.11.6480-6488.1992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro1989 · 131 citations
  2. 2trans rescue of a mutant poliovirus RNA polymerase function1991 · 49 citations
  3. 3Genetic complementation among poliovirus mutants derived from an infectious cDNA clone1986 · 129 citations
  4. 4Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis1991 · 113 citations
  5. 5Poliovirus Mutant That Does Not Selectively Inhibit Host Cell Protein Synthesis1985 · 89 citations