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February 1, 1996AJP Heart and Circulatory Physiology

Impact of extracellular buffer composition on cardioprotective efficacy of Na+/H+ exchanger inhibitors

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Population

Isolated rat hearts perfused at 37 degrees C, 75 mmHg, subjected to 20 min of global zero-flow ischemia and…

Comparison

Na+/H+ exchanger inhibitors [5-amiloride… vs Control isolated rat hearts receiving no…

Design

Preclinical

Follow-up

45 min of reperfusion

Authors

YSYasuyuki ShimadaDHDavid J. HearseMAMetin Avkiran

Discussion

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Overview

NHE inhibitor reperfusion protection may be overestimated in HEPES buffer; leaves open efficacy under physiological bicarbonate conditions in vivo.

Structured PICO

P
Population
Isolated rat hearts (n = 8/group) perfused at 37 degrees C, 75 mmHg, subjected to 20 min of global zero-flow ischemia and 45 min of reperfusion
I
Intervention
Na+/H+ exchanger inhibitors [5-(N,N-dimethyl)amiloride (DMA) or (3-methylsulfonyl-4-piperidinobenzoyl)guanidine methanesulfonate (HOE-694), 10 mumol/l] infused transiently (5 min) immediately before ischemia, during initial reperfusion, or during both periods, in bicarbonate- or N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered medium
C
Comparator
Control isolated rat hearts receiving no inhibitor in the respective buffer mediums
O
Outcome
Postischemic recovery of left ventricular developed pressure (LVDP)surrogate

The cardioprotective efficacy of Na+/H+ exchanger inhibitors during reperfusion is significantly modulated by perfusate buffer composition, suggesting efficacy may be overestimated under bicarbonate-free conditions.

Cite This Study

Shimada et al. (1996) studied this question.

synapsesocial.com/papers/6a237a3d96b50e6ae79ece73https://doi.org/10.1152/ajpheart.1996.270.2.h692
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