Key result
Ouabain superfusion initially induced acetylcholine-like effects decreasing automaticity, followed by direct effects that increased automaticity and induced tachyarrhythmias in human atrial fibers.
Why the study?
Does ouabain alter the cellular electrophysiologic properties of human atrial fibers?
Population
Human atrial fibers (HAF) obtained from patients with congenital heart disease undergoing open heart surgery
Comparison
Superfusion with ouabain (2 X 10 M) for 15 to 90… vs Baseline/control state or atropine pre-treatment
Design
Preclinical
Follow-up
up to 90 minutes
Authors
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Prolonged ouabain exposure may promote atrial tachyarrhythmias; extends ex vivo human data but leaves clinical translation open.
Does ouabain alter the cellular electrophysiologic properties of human atrial fibers?
Digitalis exerts biphasic electrophysiologic effects on human atrial fibers, with early indirect vagomimetic effects and late direct arrhythmogenic effects.
Hordof et al. (1978) studied Congenital heart disease. Ouabain was evaluated on Cellular electrophysiologic effects (maximum diastolic potential, action potential amplitude, Vmax, action potential duration, automaticity). Ouabain superfusion initially induced acetylcholine-like effects decreasing automaticity, followed by direct effects that increased automaticity and induced tachyarrhythmias in human atrial fibers.
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