Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
January 1, 1995AJP Cell Physiology

Modulation of the delayed rectifier K+ current in neurons by an angiotensin II type 2 receptor fragment

View Full Paper
Ask AI
Bookmark
Share

Key result

Intracellular injection of PEP-22 elicited an increase in delayed rectifier K+ current in cultured rat neurons similar to ANG II, an effect abolished by pertussis toxin and okadaic acid.

Population

Neurons cultured from rat hypothalamus and brain stem

Comparison

Intracellular injection of a 22-amino acid… vs Intracellular injection of a random peptide or…

Design

Preclinical

Authors

JKJian KangGansu Provincial HospitalERElaine M. RichardsUniversity of FloridaPPPhilip PosnerFlorida State University

Discussion

Loading...

Member takes

Implication

Validates cloned AT2 signaling in neurons; leaves open relevance to cardiovascular autonomic control.

Structured PICO

P
Population
Neurons cultured from rat hypothalamus and brain stem
I
Intervention
Intracellular injection of a 22-amino acid peptide (PEP-22) corresponding to the putative third intracellular loop of the cloned AT2 receptor
C
Comparator
Intracellular injection of a random peptide or normal pipette solution
O
Outcome
Delayed rectifier K+ current (IK)surrogate

This study provides direct evidence linking the cloned AT2 receptor to the modulation of delayed rectifier K+ current in neurons via Gi protein and PP2A.

Cite This Study

Kang et al. (1995) studied Neurons cultured from rat hypothalamus and brain stem. Intracellular injection of PEP-22 (22-amino acid peptide) vs. Random peptide or normal pipette solution was evaluated on Delayed rectifier K+ current (IK). Intracellular injection of PEP-22 elicited an increase in delayed rectifier K+ current in cultured rat neurons similar to ANG II, an effect abolished by pertussis toxin and okadaic acid.

synapsesocial.com/papers/6a63ef866c350a74a6f2d5cahttps://doi.org/10.1152/ajpcell.1995.268.1.c278
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition.1993 · 716 citations
  2. 2Angiotensin II receptor subtypes are coupled with distinct signal-transduction mechanisms in neurons and astrocytes from rat brain.1991 · 245 citations
  3. 3Functions of Angiotensin in the Central Nervous System1987 · 727 citations
  4. 4Amino acids 356–372 constitute a G<sub>i</sub>‐activator sequence of the α<sub>2</sub>‐adrenergic receptor and have a Phe substitute in the G protein‐activator sequence motif1992 · 70 citations
  5. 5Differential distribution of AT1 and AT2 angiotensin II receptor subtypes in the rat brain during development.1991 · 182 citations