Response of normoxic pulmonary arteries of the rat in the resting and contracted state to NO synthase blockade
Key result
Addition of L-NAME produced a contraction of 0.64 +/- 0.09 mN mm-1 in resting rat pulmonary arteries, which was absent upon endothelium removal, supporting active basal release of nitric oxide.
Population
Pulmonary arteries dissected from male Wistar-Kyoto rats (n=21)
Comparison
NG-nitro-L-arginine methyl ester 100 microM… vs Baseline resting state, endothelium-denuded…
Design
Preclinical
Authors
Discussion
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Implication
Animal findings on pulmonary NO release do not guide therapy; leaves open its role in human basal tone.
Structured PICO
P
Population
21 male Wistar-Kyoto rats used to study the release of nitric oxide in isolated pulmonary arteries.
I
Intervention
NG-nitro-L-arginine methyl ester (L-NAME) 100 microM added to myograph bath
C
Comparator
Baseline resting state, endothelium-denuded vessels, and vessels without L-NAME
O
Outcome
Vessel contraction/tension measured in mN mm-1surrogate
This preclinical study provides evidence that the active release of nitric oxide maintains resting tone in rat pulmonary arteries.
Cite This Study
Steeds et al. (1997) studied Normoxic pulmonary arteries (n=21). L-NAME vs. Absence of L-NAME / endothelium removal was evaluated on Contraction of resting pulmonary arteries. Addition of L-NAME produced a contraction of 0.64 +/- 0.09 mN mm-1 in resting rat pulmonary arteries, which was absent upon endothelium removal, supporting active basal release of nitric oxide.