Key result
Brefeldin A completely inhibited poliovirus RNA synthesis and reduced poliovirus replication by 10(5)- to 10(6)-fold, likely by preventing the formation of membranous vesicles.
Why the study?
Does brefeldin A inhibit poliovirus replication in infected cells?
Does brefeldin A inhibit poliovirus replication in infected cells?
Effect estimate: 10(5)- to 10(6)-fold inhibition
Brefeldin A profoundly inhibits poliovirus replication by blocking viral RNA synthesis, likely by preventing the formation of specific membranous vesicles.
Brefeldin A may inhibit poliovirus in cell models; leaves open therapeutic translation pending in vivo validation.
Brefeldin A (BFA), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit poliovirus replication 10(5)- to 10(6)-fold. BFA does not inhibit entry of poliovirus into the cell or translation of viral RNA. Poliovirus RNA synthesis, however, is completely inhibited by BFA. A specific class of membranous vesicles, with which the poliovirus replication complex is physically associated, is known to proliferate in poliovirus-infected cells. BFA may inhibit poliovirus replication by preventing the formation of these vesicles.
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Maynell et al. (1992) studied Poliovirus infection. Brefeldin A (BFA) was evaluated on Poliovirus replication and RNA synthesis (10(5)- to 10(6)-fold inhibition). Brefeldin A completely inhibited poliovirus RNA synthesis and reduced poliovirus replication by 10(5)- to 10(6)-fold, likely by preventing the formation of membranous vesicles.
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