Key result
A high salt diet, independent of blood pressure, decreased NO levels and DDAH/eNOS expression and activity in Dahl salt-sensitive rats.
Why the study?
Does a high salt diet affect the PRMT/ADMA/DDAH pathway and endothelial function independent of blood pressure in Dahl salt-sensitive rats?
Does a high salt diet affect the PRMT/ADMA/DDAH pathway and endothelial function independent of blood pressure in Dahl salt-sensitive rats?
High salt intake induces endothelial dysfunction via the PRMT-1/ADMA/DDAH pathway independently of blood pressure elevation in a salt-sensitive rat model.
Hypothesis-generating for BP-independent endothelial effects of salt via PRMT/ADMA/DDAH; requires human validation before clinical relevance.
Endothelial dysfunction participates in the development and progression of salt-sensitive hypertension. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase (NOS). The objectives of this study were to investigate the impact of a high salt diet on the PRMT/ADMA/DDAH (protein arginine methyltransferases; dimethylarginine dimethylaminohydrolase) pathway in Dahl salt-sensitive (DS) rats and SS-13BN consomic (DR) rats, and to explore the mechanisms that regulate ADMA metabolism independent of blood pressure reduction. Plasma levels of nitric oxide (NO) in DS rats given a high salt diet and subjected to intragastric administration of hydralazine (SH + HYD group) were lower than those given a normal salt diet (SN group). There were significant decreases in expression and activity of dimethylarginine dimethylaminohydrolase (DDAH) and endothelial NO synthase (eNOS) in DS rats given a high diet (SH group) in comparison to the SN group. The activity of DDAH and expression of eNOS in the SH + HYD group decreased more significantly than SN group. The mRNA expression of DDAH-1 and DDAH-2 were lowest in the SH group. The results suggest that salt, independent of blood pressure, can affect the PRMT-1/ADMA/DDAH system to a certain degree and lead to endothelial dysfunction in Dahl salt-sensitive rats.
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Cao et al. (2013) studied Salt-sensitive hypertension. High salt diet vs. Normal salt diet was evaluated on Plasma levels of NO, expression and activity of DDAH and eNOS. A high salt diet, independent of blood pressure, decreased NO levels and DDAH/eNOS expression and activity in Dahl salt-sensitive rats.
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