Key result
Pretreatment of intact human platelets or platelet membranes with N-ethylmaleimide abolished the alpha-adrenoceptor-mediated inhibition of adenylate cyclase by epinephrine.
Population
Human intact platelets and platelet membranes
Comparison
Pretreatment with N-ethylmaleimide (NEM) vs No NEM pretreatment
Design
Preclinical
Authors
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NEM distinguishes alpha-adrenergic from PGE1 regulation of platelet adenylate cyclase; leaves open the sulfhydryl target in inhibitory G-protein coupling.
The study demonstrates that NEM selectively uncouples alpha-adrenoceptor-mediated inhibition of platelet adenylate cyclase, indicating distinct regulatory sites for hormonal activation and inhibition.
Jakobs et al. (1982) studied this question. N-ethylmaleimide (NEM) was evaluated on Inhibition of adenylate cyclase and stimulation of GTPase by epinephrine. Pretreatment of intact human platelets or platelet membranes with N-ethylmaleimide abolished the alpha-adrenoceptor-mediated inhibition of adenylate cyclase by epinephrine.
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