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December 1, 2000Cardiovascular ResearchOpen Access

Positive inotropic effects of NO donors in isolated guinea-pig and human cardiomyocytes independent of NO species and cyclic nucleotides

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Key result

NO donors such as GSNO and DEA/NO produced significant positive inotropic effects in isolated guinea-pig and human cardiomyocytes (e.g., 48% increase with GSNO in human myocytes, P<0.05).

Population

Isolated guinea-pig and human cardiomyocytes prepared by enzymatic digestion

Comparison

NO donors including S-nitrosoglutathione and… vs Control conditions or presence of inhibitors

Design

Preclinical

Authors

DSDavid Sarkar

Discussion

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Overview

NO donor kinetics may influence isolated myocyte contractility; leaves open translation to clinical cardiac effects.

Structured PICO

P
Population
Isolated guinea-pig and human cardiomyocytes prepared by enzymatic digestion
I
Intervention
NO donors including S-nitrosoglutathione (GSNO) and diethylamine/NO (DEA/NO) at 10(-5) M, with or without isoprenaline
C
Comparator
Control conditions or presence of inhibitors (oxyhaemoglobin, ODQ, Rp-cAMPS, thapsigargin)
O
Outcome
Paced contractile amplitude at 37 degrees Csurrogate

Main Result

p-value: p=<0.05

Certain NO donors exert a positive inotropic effect on isolated guinea-pig and human cardiomyocytes that is independent of sGC and cAMP pathways.

Cite This Study

David Sarkar (2000) studied Isolated cardiomyocytes. NO donors (GSNO and DEA/NO) was evaluated on Contraction amplitude (p=<0.05). NO donors such as GSNO and DEA/NO produced significant positive inotropic effects in isolated guinea-pig and human cardiomyocytes (e.g., 48% increase with GSNO in human myocytes, P<0.05).

synapsesocial.com/papers/6a6d3d8535aa2c282cdfd67fhttps://doi.org/10.1016/s0008-6363(00)00202-9
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