Differential effects of saturated and unsaturated fatty acids on vascular reactivity in isolated mesenteric and femoral arteries of rats

Figures

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  Fig. 1. Effect of saturated fatty acid on phenylephrine (PhE)-induced contractile response in mesenteric arteries (MAs) and deep femoral arteries (DFAs) of rat. Original trace of the dose-dependent response curve of PhE in control isolated MAs and DFAs. Representative trace of PhE-induced contraction in MAs (A, B) and DFAs (D, E) with and without palmitic acid. After 30 min of exposure to physiological salt solution with or without palmitic acid, dose-response curves of different PhE concentrations (0.05 µM, 0.1 µM, 1 µM, 2 µM, 5 µM, and 10 µM) are shown. Summaries of PhE dose-response curve in MAs (C) and DFAs (F) are presented as means ± standard error of the mean. Endo, endothelial cells; ACh, acetylcholine. *p < 0.05, **p < 0.01, ***p < 0.001.

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  Fig. 2. Effect of polyunsaturated fatty acid on phenylephrine (PhE)-induced contractile response in mesenteric arteries (MAs) and deep femoral arteries (DFAs) of rat. Original trace of the dose-dependent response curve of PhE in control isolated MAs and DFAs. Representative trace of PhE-induced contraction in MAs (A, B) and DFAs (D, E) with and without linoleic acid. After 30 min of exposure to physiological salt solution with or without palmitic acid, dose-response curves of different PhE concentrations (0.05 µM, 0.1 µM, 1 µM, 2 µM, 5 µM, and 10 µM) are shown. Summaries of PhE dose-response curve in MAs (C) and DFA (F) are presented as mean ± standard error of the mean. Endo, endothelial cells; ACh, acetylcholine. ***p < 0.001.

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  Fig. 3. Effect of monounsaturated fatty acid on phenylephrine (PhE)-induced contractile response in mesenteric arteries (MAs) and deep femoral arteries (DFAs) of rat. Original trace of the dose-dependent response curve of PhE in control isolated MAs and DFAs. Representative trace of PhE-induced contraction in MAs (A, B) and DFAs (D, E) with and without oleic acid. After 30 min of exposure to physiological salt solution with or without palmitic acid, dose-response curves of different PhE concentrations (0.05 µM, 0.1 µM, 1 µM, 2 µM, 5 µM, and 10 µM) are shown. Summaries of PhE dose-response curve in MAs (C) and DFA (F) of PhE are presented as mean ± standard error of the mean. Endo, endothelial cells; ACh, acetylcholine. *p < 0.05, **p < 0.01, ***p < 0.001.

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  Fig. 4. Effect of fatty acids on the contractile response induced by high K+ in mesenteric arteries (MAs) and deep femoral arteries (DFAs) of rat. The contractile response to 40 mM of KCl in the presence or absence of palmitic acid (PA), linoleic acid (LA), oleic acid (OA) in MAs (A) and DFAs (B). *p < 0.05, **p < 0.01, ***p < 0.001.