Key result
RNA synthesized in vitro from a full-length cDNA plasmid of foot-and-mouth disease virus produced infectious particles in BHK-21 cells, primary bovine kidney cells, and baby mice.
Population
BHK-21 cells, primary bovine kidney cells, baby mice, yeast cells, and Escherichia coli
Comparison
RNA synthesized in vitro from a full-length cDNA… vs Constructs with homopolymeric sequences of up to…
Design
Preclinical
Authors
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Enables synthetic FMDV rescue across systems; leaves open extension to vaccines or other picornaviruses.
A full-length cDNA clone of foot-and-mouth disease virus was successfully constructed and shown to produce infectious RNA, demonstrating that a poly(C) tract of at least 32 nucleotides is required for viral replication.
Zibert et al. (1990) studied Foot-and-mouth disease virus. Full-length cDNA plasmid of foot-and-mouth disease virus was evaluated on Production of infectious particles and virus replication. RNA synthesized in vitro from a full-length cDNA plasmid of foot-and-mouth disease virus produced infectious particles in BHK-21 cells, primary bovine kidney cells, and baby mice.
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