Key result
Direct inoculation of suckling mice with in vitro-transcribed naked foot-and-mouth disease virus RNA, including a mutant transcript noninfectious in cell culture, resulted in the recovery of infectious virus.
Population
suckling mice
Design
Preclinical
Authors
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Provides in vivo model for FMDV RNA virulence bypassing cell culture; leaves open validation in natural hosts.
Direct inoculation of suckling mice with FMDV RNA provides a useful in vivo model for virulence studies, bypassing cell culture variant selection.
Baranowski et al. (2003) studied Foot-and-mouth disease virus (FMDV) infection. Direct inoculation with in vitro-transcribed naked FMDV RNA vs. Virion-extracted RNA was evaluated on Infectivity and recovery of infectious virus. Direct inoculation of suckling mice with in vitro-transcribed naked foot-and-mouth disease virus RNA, including a mutant transcript noninfectious in cell culture, resulted in the recovery of infectious virus.
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