Key result
Caffeine and procaine depolarized the membrane and suppressed mechanical responses in rabbit pulmonary artery smooth muscle cells, acting on both intracellular calcium stores and the surface membrane.
Population
Albino rabbits of either sex, weighing 2.5 to 3.0 kg (smooth muscle cells of the main pulmonary artery).
Comparison
Caffeine and procaine applied in vitro. vs Krebs solution (control).
Design
Preclinical
Authors
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No clinical implications for vascular tone; leaves open in vivo translation of calcium-handling mechanisms.
Caffeine and procaine act on both Ca-stored sites and the surface membrane of vascular smooth muscle cells, with non-competitive actions suggesting differences from striated muscle internal membrane structures.
Ito et al. (1977) studied Healthy animal tissue (rabbit pulmonary artery). Caffeine and Procaine vs. Krebs solution was evaluated on Membrane potential and mechanical contraction. Caffeine and procaine depolarized the membrane and suppressed mechanical responses in rabbit pulmonary artery smooth muscle cells, acting on both intracellular calcium stores and the surface membrane.
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