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March 14, 1988FEBS LettersOpen Access

The basic mechanism of stimulus-secretion coupling for catecholamine release in PC-12 cells does not absolutely require a regulated protein phosphorylation- or calmodulin-dependent step.

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Population

PC-12 cells (including mutant PC-12 cells lacking cAMP-dependent protein kinase with down-regulated protein…

Design

Preclinical

Key result

The basic mechanism of stimulus-secretion coupling for catecholamine release in PC-12 cells does not absolutely require a regulated protein phosphorylation- or calmodulin-dependent step.

Authors

HMH MatthiesHPH. Clive PalfreyRMRichard J. Miller

Discussion

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Overview

Questions necessity of phosphorylation- and calmodulin-dependent steps in exocytosis; leaves open applicability beyond PC-12 cells.

Structured PICO

P
Population
PC-12 cells (including mutant PC-12 cells lacking cAMP-dependent protein kinase (PK-A) with down-regulated protein kinase C (PK-C), and digitonin-permeabilized PC-12 cells)
I
Intervention
Agents that increase intracellular Ca2+, phorbol esters, agents elevating cAMP, calmodulin (CaM) inhibitors, Mn2+, and Ba2+
O
Outcome
Catecholamine release/secretionsurrogate

The basic mechanism of stimulus-secretion coupling in PC-12 cells does not absolutely require protein phosphorylation or calmodulin-dependent steps.

Cite This Study

Matthies et al. (1988) studied this question. Intracellular Ca2+ elevating agents, cAMP, phorbol esters, and CaM inhibitors was evaluated on Catecholamine release. The basic mechanism of stimulus-secretion coupling for catecholamine release in PC-12 cells does not absolutely require a regulated protein phosphorylation- or calmodulin-dependent step.

synapsesocial.com/papers/6a6e92dc26770c2b8ddfc5dahttps://doi.org/10.1016/0014-5793(88)81132-3
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inhibition of Ca2+ channels via alpha 2-adrenergic and muscarinic receptors in pheochromocytoma (PC-12) cells1991 · 30 citations
  2. 2Roles of Ca2+ influx through ATP-activated channels in catecholamine release from pheochromocytoma PC12 cells1992 · 45 citations
  3. 3Calmodulin Modulates Mitogen‐Activated Protein Kinase Activation in Response to Membrane Depolarization in PC12 Cells1998 · 34 citations
  4. 4Calcium inflow-dependent protein kinase C activity is involved in the modulation of transmitter release in the neuromuscular junction of the adult rat2005 · 37 citations
  5. 5Exposure to cAMP and β‐adrenergic stimulation recruits Ca<sub>V</sub>3 T‐type channels in rat chromaffin cells through Epac cAMP‐receptor proteins2004 · 79 citations