Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
May 11, 2004The Journal of PhysiologyOpen Access

Exposure to pCPT-cAMP for 3-5 days induced high density of low-voltage-activated T-type channels in RCCs, an effect mimicked by Epac agonist and isoprenaline, and prevented by protein synthesis inhibitor anisomycin.

View Full Paper
Ask AI
Bookmark
Share

Population

Adult rat chromaffin cells (RCCs) grown in serum-free medium

Comparison

Exposure to pCPT-cAMP, forskolin, IBMX… vs Untreated cells or cells treated with inhibitors

Design

Preclinical

Follow-up

3-5 days

Authors

MNMonica NovaraPBPietro BaldelliDCDaniela Cavallari

Discussion

Loading...

Member takes

Overview

May boost chromaffin excitability during sustained sympathetic drive; hypothesis-generating for Epac-T-type roles in human cardiovascular disease.

Structured PICO

P
Population
Adult rat chromaffin cells (RCCs) grown in serum-free medium
I
Intervention
Exposure to pCPT-cAMP (200 microM), forskolin, IBMX, isoprenaline, or Epac agonist 8CPT-2Me-cAMP for 3-5 days
C
Comparator
Untreated cells or cells treated with inhibitors (anisomycin, H89, Rp-cAMPS)
O
Outcome
Expression and density of low-voltage-activated T-type channelssurrogate

cAMP and beta-adrenergic stimulation recruit T-type calcium channels in rat chromaffin cells via Epac-dependent pathways, potentially increasing cell excitability during sustained sympathetic stimulation.

Cite This Study

Novara et al. (2004) studied this question.

synapsesocial.com/papers/6a7c83034ece96453776de5chttps://doi.org/10.1113/jphysiol.2004.061184
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Spatiotemporal Dynamics of β-Adrenergic cAMP Signals and L-Type Ca <sup>2+</sup> Channel Regulation in Adult Rat Ventricular Myocytes2008 · 157 citations
  2. 2Depotentiation of intact rat cardiac muscle unmasks an Epac‐dependent increase in myofilament Ca<sup>2+</sup> sensitivity2015 · 12 citations
  3. 3Localized cAMP-dependent signaling mediates beta 2-adrenergic modulation of cardiac excitation-contraction coupling1997 · 103 citations
  4. 4The cAMP binding protein Epac modulates Ca 2+ sparks by a Ca 2+ /calmodulin kinase signalling pathway in rat cardiac myocytes2007 · 212 citations
  5. 5Co-expression of a Ca2+-inhibitable Adenylyl Cyclase and of a Ca2+-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney1998 · 101 citations