Key result
A pan-serotype lateral flow strip test detected all seven FMDV serotypes with diagnostic sensitivity comparable to AgELISA and 100% diagnostic specificity without cross-reactivity.
Why the study?
Current laboratory methods for detecting foot-and-mouth disease virus are labor-intensive and require trained personnel and specialized equipment, highlighting the need for rapid detection methods.
Does a pan-serotype lateral flow strip test accurately detect FMDV compared to AgELISA?
Does a pan-serotype lateral flow strip test accurately detect FMDV compared to AgELISA?
A newly developed pan-serotype lateral flow strip test provides rapid, highly specific, and sensitive on-site detection of all seven FMDV serotypes.
Answers are grounded in this paper and cite their sources.
May enable rapid field FMDV detection; leaves open prospective validation against reference standards before adoption.
Foot-and-mouth disease virus (FMDV) causes FMD, a highly contagious disease of cloven-hoofed animals including cattle, goats, pigs and sheep. Rapid detection of FMDV is critical to limit the devastating economic losses due to FMD. Current laboratory methods for FMDV detection such as virus isolation, real-time reverse transcription PCR and antigen detection enzyme-linked immunosorbent assay (AgELISA) are labor-intensive, requiring trained personnel and specialized equipment. We present the development and validation of a pan-serotype lateral flow strip test (LFST) that uses recombinant bovine integrin αvβ6 as a universal capture ligand and a pan-serotype monoclonal antibody (mAb) to detect FMDV. The LFST detected all seven FMDV serotypes, where the diagnostic sensitivity was comparable to the AgELISA, and the diagnostic specificity was 100% without cross-reactivity to other viruses causing vesicular disease in livestock. This rapid test will be useful for on-site FMDV detection, as well as in laboratories in endemic countries where laboratory resources are limited.
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Yang et al. (2022) studied Foot-and-mouth disease virus (FMDV). Pan-serotype lateral flow strip test (LFST) vs. Antigen detection enzyme-linked immunosorbent assay (AgELISA) was evaluated on Diagnostic sensitivity and specificity. A pan-serotype lateral flow strip test detected all seven FMDV serotypes with diagnostic sensitivity comparable to AgELISA and 100% diagnostic specificity without cross-reactivity.
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