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January 1, 1988Nucleic Acids ResearchOpen Access

Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems

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Population

Primary cultures derived from mouse skeletal muscle, myogenic cell lines, and non-myogenic 3T6 and CV1 cells

Comparison

Transfection with proximal upstream flanking… vs Comparison between different cell types and…

Design

Preclinical

Authors

PDPhilippe DaubasAKAndré KlarsfeldIGIan Garner

Discussion

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Overview

Demonstrates dual-promoter control of myosin light chain in murine myogenesis; leaves open relevance to human cardiac development or disease.

Structured PICO

P
Population
Primary cultures derived from mouse skeletal muscle, myogenic cell lines, and non-myogenic 3T6 and CV1 cells
I
Intervention
Transfection with proximal upstream flanking sequences of mouse myosin alkali light chain gene (MLC1F/MLC3F), mouse alpha-cardiac actin gene, and chicken acetylcholine receptor alpha-subunit gene linked to the bacterial chloramphenicol acetyl transferase (CAT) gene
C
Comparator
Comparison between different cell types (myotubes vs myoblasts vs non-myogenic cells) and different promoter constructs
O
Outcome
CAT activity (gene expression)surrogate

This basic science study demonstrates that the mouse myosin alkali light chain gene has two functional promoters with developmentally regulated expression in primary muscle cultures.

Cite This Study

Daubas et al. (1988) studied this question.

synapsesocial.com/papers/6a6f92c8439bab0cabc2d7f3https://doi.org/10.1093/nar/16.4.1251
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Upstream Regions of the Human Cardiac Actin Gene That Modulate Its Transcription in Muscle Cells: Presence of an Evolutionarily Conserved Repeated Motif1986 · 155 citations
  2. 2Two-Level Regulation of Cardiac Actin Gene Transcription: Muscle-Specific Modulating Factors Can Accumulate before Gene Activation1986 · 55 citations
  3. 3Complex Regulation of the Muscle-Specific Contractile Protein (Troponin I) Gene1987 · 36 citations
  4. 4Effects of chlorpromazine and phencyclidine on mouse C2 acetylcholine receptor kinetics.1986 · 82 citations
  5. 5A 5′-Flanking Region of the Chicken Acetylcholine Receptor α-Subunit Gene Confers Tissue Specificity and Developmental Control of Expression in Transfected Cells1987 · 90 citations