What question did this study set out to answer?

This study aims to investigate the role of nitric oxide in endothelium-dependent relaxation in hypertensive vs. control rats.

June 1, 1991HypertensionOpen Access

Effect of NG-monomethyl L-arginine on endothelium-dependent relaxation in arterioles of one-kidney, one clip hypertensive rats.

View Full Paper

Ask AI

Bookmark

Why the study?

Does NG-monomethyl L-arginine alter endothelium-dependent relaxation in arterioles of one-kidney, one clip hypertensive rats compared to controls?

Population

Control (n = 9) and one-kidney, one clip hypertensive (1K1C) rats (n = 11) of 4 weeks' duration

Comparison

Superfusion with NG-monomethyl L-arginine (10 M)… vs Control rats and baseline measurements before…

Design

Preclinical

Authors

TNT. NakamuraKyoto Women's University RPRussell L. PrewittLouisiana State University

Discussion

Member takes

Implication

Impaired NO-mediated arteriolar responses in 1K1C rats; leaves open mechanistic role of nitric oxide in hypertension pending higher-evidence studies.

Key Points

This study aims to investigate the role of nitric oxide in endothelium-dependent relaxation in hypertensive vs. control rats.
Used intravital microscopy to assess dose-response curves to acetylcholine, bradykinin, and nitroprusside in arterioles.
Applied L-NMMA and indomethacin during observations.
Compared responses between control and one-kidney, one-clip hypertensive rats.
Resting arteriolar tone was higher in one-kidney, one-clip (1K1C) rats than in controls (p<0.05).
L-NMMA increased arteriolar tone and inhibited vasodilation in controls (p<0.05) but not in 1K1C rats.
Indomethacin with L-NMMA further reduced responses to bradykinin in both groups.

Structured PICO

Does NG-monomethyl L-arginine alter endothelium-dependent relaxation in arterioles of one-kidney, one clip hypertensive rats compared to controls?

Population

Control (n = 9) and one-kidney, one clip hypertensive (1K1C) rats (n = 11) of 4 weeks' duration

Intervention

Superfusion with NG-monomethyl L-arginine (LNMMA) (10(-4) M) and both LNMMA (10(-4) M) and indomethacin (2.8 x 10(-5) M)

Comparator

Control rats and baseline measurements before superfusion

Outcome

Dose-response curves (vasodilation/arteriolar tone) to topically applied acetylcholine, bradykinin, and nitroprusside obtained by intravital microscopysurrogate

Resting arteriolar tone is increased and endothelium-dependent vasodilation is attenuated in 1K1C hypertensive rats, likely due to decreased basal release or synthesis of nitric oxide.

Cite This Study

Nakamura et al. (1991) studied this question.

synapsesocial.com/papers/6a6f99f92fdcb5703edb4b8f https://doi.org/10.1161/01.hyp.17.6.875

View Full Paper

Ask AI

Bookmark

Implication

Impaired NO-mediated arteriolar responses in 1K1C rats; leaves open mechanistic role of nitric oxide in hypertension pending higher-evidence studies.

Effect of NG-monomethyl L-arginine on endothelium-dependent relaxation in arterioles of one-kidney, one clip hypertensive rats.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of NG-monomethyl L-arginine on endothelium-dependent relaxation in arterioles of one-kidney, one clip hypertensive rats.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider