2-Aminopurine Selectively Inhibits Splicing of Tumor Necrosis Factor Alpha mRNA

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Population

Primary human lymphoid cells, BHK-21 cells, and HL-60 cells

Comparison

2-Aminopurine, typically at 10 mM concentration vs Untreated control cells or cells treated with…

Design

Preclinical

Authors

Discussion

Hypothesis-generating for post-transcriptional TNF-alpha control; leaves open therapeutic relevance pending human validation.

Population

Primary human lymphoid cells (peripheral blood mononuclear cells from healthy donors), BHK-21 cells, and HL-60 cells

Intervention

2-Aminopurine (2-AP), typically at 10 mM concentration

Comparator

Untreated control cells or cells treated with other inhibitors (e.g., pentoxifylline, H7, staurosporine)

Outcome

Expression and splicing of tumor necrosis factor alpha (TNF-alpha) mRNA

2-Aminopurine selectively blocks the splicing of TNF-alpha mRNA, revealing a post-transcriptional regulatory mechanism for this cytokine.

Jarrous et al. (1996) studied this question.

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