What question did this study set out to answer?

To test whether insulin rapidly recruits skeletal muscle microvasculature in humans and whether expanding microvascular volume enhances muscle insulin clearance.

August 25, 2007DiabetesOpen Access

Hyperinsulinemia Rapidly Increases Human Muscle Microvascular Perfusion but Fails to Increase Muscle Insulin Clearance

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Why the study?

Does hyperinsulinemia increase muscle microvascular perfusion and insulin clearance in lean, healthy volunteers?

Population

14 lean, healthy volunteers

Comparison

2-h hyperinsulinemic-euglycemic clamp (1 mU x kg… vs Basal state (before clamp)

Design

Other

Follow-up

2 hours

Authors

EEEmma EgglestonWest Virginia University LJLinda A. JahnUniversity Hospital Carl Gustav Carus EBEugene J. BarrettUniversity of Pisa

Discussion

Member takes

Implication

No immediate clinical implications in healthy volunteers; leaves open relevance of microvascular insulin dynamics to resistance in diabetes.

Key Points

To test whether insulin rapidly recruits skeletal muscle microvasculature in humans and whether expanding microvascular volume enhances muscle insulin clearance.
Conducted a 2-hour hyperinsulinemic-euglycemic clamp (1 mU·kg⁻¹·min⁻¹) in 14 lean, healthy volunteers.
Measured forearm muscle microvascular volume via contrast-enhanced ultrasound alongside total forearm blood flow and muscle insulin and glucose uptake using the forearm balance method.
Forearm muscle microvascular volume increased significantly within 20 minutes of insulin infusion (P < 0.01), while total forearm blood flow did not increase until 100 minutes.
Forearm insulin uptake rose from 15 ± 3 fmol·min⁻¹·(100 ml)⁻¹ at baseline to 87 ± 13 fmol·min⁻¹·(100 ml)⁻¹ during the final 40 minutes of the clamp (P < 0.001).
Both the extraction fraction and clearance of insulin declined significantly during physiological hyperinsulinemia (P = 0.02 for each).

Structured PICO

Does hyperinsulinemia increase muscle microvascular perfusion and insulin clearance in lean, healthy volunteers?

Population

14 lean, healthy volunteers

Intervention

2-h hyperinsulinemic-euglycemic clamp (1 mU x kg(-1) x min(-1))

Comparator

Basal state (before clamp)

Outcome

Microvascular volume, total blood flow, and muscle insulin and glucose uptakesurrogate

Insulin rapidly expands human muscle microvascular volume but decreases muscle insulin clearance, suggesting a saturable transendothelial transport mechanism.

Cite This Study

Eggleston et al. (2007) studied this question.

synapsesocial.com/papers/6a7026fc31a3df8243286e44 https://doi.org/10.2337/db07-0670

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August 25, 2007DiabetesOpen Access

Hyperinsulinemia Rapidly Increases Human Muscle Microvascular Perfusion but Fails to Increase Muscle Insulin Clearance

View Full Paper

Ask AI

Bookmark

Why the study?

Does hyperinsulinemia increase muscle microvascular perfusion and insulin clearance in lean, healthy volunteers?

Population

14 lean, healthy volunteers

Comparison

2-h hyperinsulinemic-euglycemic clamp (1 mU x kg… vs Basal state (before clamp)

Design

Other

Follow-up

2 hours

Authors

EEEmma EgglestonWest Virginia University LJLinda A. JahnUniversity Hospital Carl Gustav Carus EBEugene J. BarrettUniversity of Pisa

Discussion

Member takes

Implication

No immediate clinical implications in healthy volunteers; leaves open relevance of microvascular insulin dynamics to resistance in diabetes.

Key Points

To test whether insulin rapidly recruits skeletal muscle microvasculature in humans and whether expanding microvascular volume enhances muscle insulin clearance.
Conducted a 2-hour hyperinsulinemic-euglycemic clamp (1 mU·kg⁻¹·min⁻¹) in 14 lean, healthy volunteers.
Measured forearm muscle microvascular volume via contrast-enhanced ultrasound alongside total forearm blood flow and muscle insulin and glucose uptake using the forearm balance method.
Forearm muscle microvascular volume increased significantly within 20 minutes of insulin infusion (P < 0.01), while total forearm blood flow did not increase until 100 minutes.
Forearm insulin uptake rose from 15 ± 3 fmol·min⁻¹·(100 ml)⁻¹ at baseline to 87 ± 13 fmol·min⁻¹·(100 ml)⁻¹ during the final 40 minutes of the clamp (P < 0.001).
Both the extraction fraction and clearance of insulin declined significantly during physiological hyperinsulinemia (P = 0.02 for each).

Structured PICO

Does hyperinsulinemia increase muscle microvascular perfusion and insulin clearance in lean, healthy volunteers?

Population

14 lean, healthy volunteers

Intervention

2-h hyperinsulinemic-euglycemic clamp (1 mU x kg(-1) x min(-1))

Comparator

Basal state (before clamp)

Outcome

Microvascular volume, total blood flow, and muscle insulin and glucose uptakesurrogate

Insulin rapidly expands human muscle microvascular volume but decreases muscle insulin clearance, suggesting a saturable transendothelial transport mechanism.

Cite This Study

Eggleston et al. (2007) studied this question.

synapsesocial.com/papers/6a7026fc31a3df8243286e44 https://doi.org/10.2337/db07-0670

Also Consider

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