March 1, 2000AJP Cell Physiology

Mechanisms of Pi regulation of the skeletal muscle SR Ca2+ release channel

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Population

Purified sarcoplasmic reticulum Ca2+ release channels, isolated SR vesicles, and skinned skeletal muscle…

Comparison

Inorganic phosphate at various concentrations vs 0 Pi (absence of Pi) or ATP analog (AMP-PCP)

Design

Preclinical

Authors

EBEdward M. BalogGeorgia Institute of Technology BFBradley R. FruenUniversity of North Carolina at Chapel Hill PKP.K. KaneUniversity of Minnesota

Discussion

Member takes

Implication

Pi may modulate skeletal muscle Ca2+ release; leaves open physiological relevance and requires validation in intact systems.

Structured PICO

Population

Purified sarcoplasmic reticulum (SR) Ca2+ release channels, isolated SR vesicles, and skinned skeletal muscle fibers

Intervention

Inorganic phosphate (Pi) at various concentrations (e.g., 20 mM, 40 mM)

Comparator

0 Pi (absence of Pi) or ATP analog (AMP-PCP)

Outcome

Single channel open probability (Po), mean channel closed time, mean channel open time, [(3)H]ryanodine binding, and Ca2+-induced Ca2+ releasesurrogate

Inorganic phosphate acts as an endogenous modulator of the skeletal muscle SR Ca2+ release channel, increasing open probability via a mechanism distinct from adenine nucleotides.

Cite This Study

Balog et al. (2000) studied this question.

synapsesocial.com/papers/6a708b168031ec7bb1dc6a3b https://doi.org/10.1152/ajpcell.2000.278.3.c601

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Mechanisms of Pi regulation of the skeletal muscle SR Ca2+ release channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mechanisms of Pi regulation of the skeletal muscle SR Ca2+ release channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider