This study identifies key regulatory elements in the human renin promoter and Intron A that control renin gene transcription in Calu-6 cells.
No clinical implications yet; leaves open cAMP's role in human renin regulation pending in vivo confirmation.
Calu-6 cells were characterized for studying the transcriptional regulation of the human renin gene. Analysis of cis-acting elements of the renin promoter showed the highest activity within the first 582 bp in serum-free conditions and of the 892 bp in the presence of serum. cAMP activates renin mRNA synthesis parallel to renin production (20-fold increase) as well renin promoter activity (2-fold). cAMP response element and the (-77 to -67) element are both necessary for activation of the renin promoter but do not act independently. Functional analysis of Intron A revealed the presence of a silencer specific to renin-producing cells.
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Germain et al. (1997) studied this question.
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