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January 1, 1997Clinical and Experimental Hypertension

Regulation of Human Renin Gene Transcription by cAMP

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Population

Chorionic cells transfected with human renin gene promoter-luciferase reporter constructs

Comparison

Forskolin treatment and mutation/deletion of CRE… vs Untreated cells or cells with wild-type promoter…

Design

Preclinical

Authors

SGStéphane GermainCentre National de la Recherche ScientifiqueTKTadashi KonoshitaUniversity of FukuiSFSébastien FuchsWestern University of Health Sciences

Discussion

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Overview

CRE mutation blunts but does not abolish forskolin-driven renin transcription; hypothesis-generating for promoter mechanisms, no clinical implications yet.

Structured PICO

P
Population
Chorionic cells transfected with human renin gene promoter-luciferase reporter constructs
I
Intervention
Forskolin treatment (cAMP induction) and mutation/deletion of CRE and Pit-1 sites
C
Comparator
Untreated cells or cells with wild-type promoter constructs
O
Outcome
Luciferase expression (reporter gene activity)surrogate

Maximal cAMP-induced transcription of the human renin gene requires both the CRE region and a Pit-1 site interacting with tissue-specific factors.

Cite This Study

Germain et al. (1997) studied this question.

synapsesocial.com/papers/6a70f1be8031ec7bb1dcad40https://doi.org/10.3109/10641969709083168
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Transcriptional induction of the human renin gene by cyclic AMP requires cyclic AMP response element-binding protein (CREB) and a factor binding a pituitary-specific <i>trans</i>-acting factor (Pit-1) motif1996 · 53 citations
  2. 2Promoter activity of human renin 5'-flanking DNA sequences is activated by the pituitary-specific transcription factor Pit-1.1993 · 39 citations
  3. 3Isolation and Characterization of Renin-Producing Human Chorionic Cells in Culture1988 · 43 citations
  4. 4Use of a specific antiserum for renin detection in human kidney.1980 · 65 citations
  5. 5Renin gene promoter activity in GC cells is regulated by cAMP and thyroid hormone through Pit-1-dependent mechanisms.1994 · 35 citations