What question did this study set out to answer?

To determine the respiratory phases during which peripheral chemoreceptors recruit pre-sympathetic C1 neurons and whether C1 neuron activation influences sympathetic activity across all phases of respiration.

June 20, 2022Acta Physiologica

Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats

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Why the study?

It was unknown in which respiratory phase(s) pre-sympathetic C1 neurones are recruited by peripheral chemoreceptors and whether C1 neurone activation affects all phases of respiratory modulation of sympathetic activity.

Population

In situ preparation of rat

Comparison

Peripheral chemoreflex stimulation vs selective optogenetic activation of C1 neurones

Design

Preclinical in situ electrophysiological and optogenetic study

Authors

MSMelina P. da SilvaUniversidade Federal de São Paulo PSPedro F. SpillerUniversidade de São Paulo JPJulian F. R. PatonGeneral Cardiology

Discussion

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Implication

C1 neurone phase recruitment during chemoreflex remains preclinical; leaves open any role in human sympathetic dysregulation.

Key Points

To determine the respiratory phases during which peripheral chemoreceptors recruit pre-sympathetic C1 neurons and whether C1 neuron activation influences sympathetic activity across all phases of respiration.
Performed intracellular recordings from baroreceptive pre-sympathetic C1 neurons during peripheral chemoreflex activation induced by potassium cyanide in an in situ rat preparation.
Selectively stimulated C1 neurons optogenetically to evaluate respiratory-phase-related modulation of sympathetic nerve activity.
Peripheral chemoreceptor activation selectively increased C1 neuron firing frequency and both the frequency and amplitude of excitatory post-synaptic currents during the expiratory phase only.
Optogenetic stimulation of C1 neurons excited inspiratory neurons and secondarily inhibited expiratory neurons while generating uniform increases in sympathetic activity across all phases of respiration.

Structured PICO

Population

in situ preparation of rat

Intervention

Activation of peripheral chemoreceptors using cytotoxic hypoxia (potassium cyanide) and optogenetic stimulation of C1 neurones

Outcome

Firing frequency of C1 neurones and frequency/amplitude of excitatory post-synaptic currents across respiratory phasessurrogate

Peripheral chemoreceptor activation excites pre-sympathetic C1 neurones exclusively during expiration, revealing novel functional organization of respiratory-sympathetic neural networks.

Cite This Study

Silva et al. (2022) studied this question.

synapsesocial.com/papers/6a71022935aa2c282ce2546c https://doi.org/10.1111/apha.13853

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Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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