What question did this study set out to answer?

This research aims to clarify the relationship between collagen matrix structure and passive ventricular mechanics in isolated rat hearts.

March 1, 1994AJP Heart and Circulatory Physiology

Contribution of collagen matrix to passive left ventricular mechanics in isolated rat hearts

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Population

16 isolated rat hearts

Comparison

Perfusion with bacterial collagenase for 60 min vs Krebs solution-treated state

Design

Preclinical

Follow-up

60 min

Authors

DMDeidre A. MacKennaRegulus Therapeutics (United States)JOJeffrey H. OmensHeart Failure / Cardiomyopathy AMAndrew D. McCullochHeart Failure & Transplant

Discussion

Member takes

Implication

Supports collagen's role in rat LV geometry; hypothesis-generating for matrix contributions to human remodeling.

Key Points

This research aims to clarify the relationship between collagen matrix structure and passive ventricular mechanics in isolated rat hearts.
Sixteen rat hearts were perfused with bacterial collagenase for 60 minutes to deplete collagen.
Quantitative morphology was assessed using picrosirius red and scanning electron microscopy to evaluate collagen area and structural integrity.
Passive pressure-volume curves were analyzed to measure changes in left ventricular volume and sarcomere lengths before and after collagen depletion.
Collagen area fraction decreased by 36%, primarily affecting medium-sized fibers.
Left ventricular volume increased significantly at all pressures (from 0.203 +/- 0.061 to 0.265 +/- 0.061 ml at 5 mmHg, P < 0.0001).
No change in strain during loading was observed, but remodeling strain showed increases in circumferential and longitudinal stretching.

Structured PICO

Population

16 isolated rat hearts

Intervention

Perfusion with bacterial collagenase for 60 min

Comparator

Krebs solution-treated state

Outcome

Passive left ventricular mechanics (ventricular volume, strain, sarcomere lengths)surrogate

Depletion of the collagen matrix in isolated rat hearts leads to increased ventricular volume and sarcomere lengths without altering ventricular compliance.

Cite This Study

MacKenna et al. (1994) studied this question.

synapsesocial.com/papers/6a711afdf44fa9f079dee067 https://doi.org/10.1152/ajpheart.1994.266.3.h1007

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Contribution of collagen matrix to passive left ventricular mechanics in isolated rat hearts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Contribution of collagen matrix to passive left ventricular mechanics in isolated rat hearts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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