What question did this study set out to answer?

To characterize the surface phenotype and quantify infiltrating T lymphocyte subpopulations during acute cardiac allograft rejection under different immunosuppressive protocols.

September 1, 1984Transplantation

Use of Monoclonal Antibodies to Quantitate T Lymphocyte Subpopulations in Human Cardiac Allografts

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Why the study?

What is the surface phenotype of T lymphocytes involved in acute rejection of human cardiac allografts under different immunosuppressive regimens?

Population

40 human cardiac biopsies

Comparison

Quantitation of T lymphocyte subpopulations… vs Comparison between rejecting vs. nonrejecting…

Design

Cross-sectional

Authors

MRMarlene L. RoseHarefield Hospital JGJ. Alastair GracieUniversity of Strathclyde AFAudrey FraserWellcome Sanger Institute

Discussion

Member takes

Implication

May support T-cell phenotyping for rejection assessment under conventional immunosuppression; leaves open cyclosporine's modulation of subsets in cardiac allografts.

Key Points

To characterize the surface phenotype and quantify infiltrating T lymphocyte subpopulations during acute cardiac allograft rejection under different immunosuppressive protocols.
Evaluated 40 frozen human cardiac biopsies (N=40), including 17 from patients on conventional immunosuppression, 18 on cyclosporine, and 5 from nontransplanted control hearts.
Quantified Leu 4 (pan-T-cell), Leu 2a (suppressor/cytotoxic), and Leu 3a (helper/inducer) positive cells using monoclonal antibodies and an immunoperoxidase technique.
Biopsies from patients on conventional therapy undergoing rejection contained 68.3 ± 10 leukocytes/0.048 mm2 with 47 ± 7.4% T cells (86% Leu 2a/T8 phenotype), compared to 43.3 ± 18.8 leukocytes/0.048 mm2 and 12.6% T cells without T8 predominance in nonrejecting grafts.
Cyclosporine-treated grafts exhibited heavy leukocyte infiltration that did not correlate with histological rejection, though rejection was accompanied by increased total T cell numbers lacking a uniform subset distribution.
Nontransplanted control biopsies contained 31.5 ± 9.4 infiltrating leukocytes/0.048 mm2 and were entirely devoid of T lymphocytes.

Structured PICO

What is the surface phenotype of T lymphocytes involved in acute rejection of human cardiac allografts under different immunosuppressive regimens?

Population

40 human cardiac biopsies (17 from patients receiving conventional immunosuppression, 18 from patients receiving cyclosporine as the major immunosuppressive drug, and 5 from nontransplanted hearts)

Intervention

Quantitation of T lymphocyte subpopulations using monoclonal antibodies (Leu 4, Leu 2a, Leu 3a) and an immunoperoxidase technique

Comparator

Comparison between rejecting vs. nonrejecting biopsies, and conventional immunosuppression vs. cyclosporine therapy

Outcome

Number and phenotype of infiltrating leukocytes and T cell subsets (Leu 4/pan-T, Leu 2a/suppressor-cytotoxic, and Leu 3a/helper-inducer positive cells)surrogate

Acute cardiac allograft rejection in patients on conventional immunosuppression is characterized by an influx of predominantly suppressor/cytotoxic (T8) T cells, a pattern that is altered by cyclosporine therapy.

Cite This Study

Rose et al. (1984) studied this question.

synapsesocial.com/papers/6a71365d2163a0a01bc56013 https://doi.org/10.1097/00007890-198409000-00007

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Implication

May support T-cell phenotyping for rejection assessment under conventional immunosuppression; leaves open cyclosporine's modulation of subsets in cardiac allografts.

Use of Monoclonal Antibodies to Quantitate T Lymphocyte Subpopulations in Human Cardiac Allografts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Use of Monoclonal Antibodies to Quantitate T Lymphocyte Subpopulations in Human Cardiac Allografts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider