January 1, 1975Hoppe-Seyler´s Zeitschrift für physiologische Chemie

Conformational Differences in Myosin, VI. Radioactive Labeling of Specific Thiol Groups as Influenced by Ligand Binding

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Population

Myosin protein

Comparison

Radioactive labeling with N-ethyl-[2,3-14C2]-male… vs Different conditions

Design

Preclinical

Authors

MSMarcus SchaubSwiss Federal Institute for Forest, Snow and Landscape Research JWJ. G. WattersonGriffith University PWPeter G. WaserUniversity of Zurich

Discussion

Member takes

Implication

No direct clinical implications from this animal study; leaves open myosin conformational dynamics as a target for future translational research.

Structured PICO

Population

Myosin protein

Intervention

Radioactive labeling with N-ethyl-[2,3-14C2]-maleimide under various conditions of temperature, ionic strength, and ligand binding

Comparator

Different conditions (e.g., KCl >0.4M, temperatures >10 degrees C, presence of magnesium nucleotide complex)

Outcome

Changes in mono- and divalent cation-stimulated ATPase activities and patterns of incorporation of N-ethyl-[14C2]-maleimidesurrogate

This basic science study reveals 9 distinct conformations of intact myosin based on thiol group reactivity and demonstrates that magnesium nucleotide complex binding induces cooperativity between its active sites.

Cite This Study

Schaub et al. (1975) studied this question.

synapsesocial.com/papers/6a71429735aa2c282ce29248 https://doi.org/10.1515/bchm2.1975.356.1.325

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Implication

No direct clinical implications from this animal study; leaves open myosin conformational dynamics as a target for future translational research.

Conformational Differences in Myosin, VI. Radioactive Labeling of Specific Thiol Groups as Influenced by Ligand Binding

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Conformational Differences in Myosin, VI. Radioactive Labeling of Specific Thiol Groups as Influenced by Ligand Binding

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider