January 1, 1995Nucleic Acids ResearchOpen Access

An efficient strategy for the synthesis of circular RNA molecules

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Population

RNA molecules (peach latent mosaic viroid (PLMVd) sequences used as an example)

Comparison

A 5-step procedure for synthesizing circular RNA… vs Conventional recombinant RNA techniques or T4…

Design

Preclinical

Authors

DBDanièle BeadudryUniversité de Sherbrooke JPJean-Pierro PerreaultUniversité de Sherbrooke

Discussion

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Implication

Simplifies circRNA production for experiments; extends lab tools for cardiovascular researchers while leaving clinical translation open.

Structured PICO

Population

RNA molecules (peach latent mosaic viroid (PLMVd) sequences used as an example)

Intervention

A 5-step procedure for synthesizing circular RNA molecules using circularly permuted RNA strategy, PCR amplification, run-off transcription, dephosphorylation-phosphorylation, and T4 RNA ligase

Comparator

Conventional recombinant RNA techniques or T4 DNA ligase strategy

Outcome

Synthesis of pure, homogeneous circular RNA molecules

A fast and simple one test-tube procedure was developed for synthesizing large amounts of pure, homogeneous circular RNA molecules.

Limitations

Requires some knowledge of the secondary structure in the region of the ligation site

Cite This Study

Beadudry et al. (1995) studied this question.

synapsesocial.com/papers/6a719e27e5469ee92be20f8b https://doi.org/10.1093/nar/23.15.3064

View Full Paper

Ask AI

Bookmark

An efficient strategy for the synthesis of circular RNA molecules

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

An efficient strategy for the synthesis of circular RNA molecules

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider