Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 1, 1985Proceedings of the National Academy of SciencesOpen Access

Inositol 1,4,5-trisphosphate: a possible chemical link in excitation-contraction coupling in muscle.

View Full Paper
Ask AI
Bookmark
Share

Population

Skeletal muscle and skinned muscle fibers

Comparison

Exogenous inositol 1,4,5-trisphosphate with or… vs Normal conditions (without blockers) or baseline

Design

Preclinical

Authors

JVJ VergaraUniversity of MindanaoRTR Y TsienUniversity of California, San DiegoMDMichael DeLayIDEXX Laboratories (United States)

Discussion

Loading...

Member takes

Overview

Supports InsP3 role in skeletal muscle Ca2+ release; hypothesis-generating and requires mammalian validation before any clinical consideration.

Key Points

  • This research aims to explore the role of inositol 1,4,5-trisphosphate in the mechanism of excitation-contraction coupling in skeletal muscle.
  • Electrical stimulation was used to induce InsP3 release in muscle fibers.
  • Exogenous InsP3 was applied to skinned muscle fibers to observe calcium release dynamics.
  • Blockers of InsP3 release were utilized to assess their impact on calcium transients during electrical stimulation.
  • InsP3 is released during electrical stimulation of muscles, facilitating calcium release.
  • At high doses, InsP3 releases calcium from muscle fibers, but only at low doses with InsP3 5-phosphatase blockers.
  • Blockers of InsP3 release effectively inhibit calcium transients triggered by electrical stimulation.

Structured PICO

P
Population
Skeletal muscle and skinned muscle fibers
I
Intervention
Exogenous inositol 1,4,5-trisphosphate (InsP3) with or without InsP3 5-phosphatase blockers, and electrical stimulation
C
Comparator
Normal conditions (without blockers) or baseline
O
Outcome
Calcium release from the sarcoplasmic reticulum and calcium transientssurrogate

InsP3 may act as a chemical second messenger linking T-tubular membrane depolarization to calcium release from the sarcoplasmic reticulum in skeletal muscle.

Cite This Study

Vergara et al. (1985) studied this question.

synapsesocial.com/papers/6a71e8f8febe604dd70a582bhttps://doi.org/10.1073/pnas.82.18.6352
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1INHIBITION BY NEOMYCIN OF POLYPHOSPHOINOSITIDE TURNOVER IN SUBCELLULAR FRACTIONS OF GUINEA‐PIG CEREBRAL CORTEX IN VITRO1976 · 181 citations
  2. 2Temperature and compositional dependence of the structure of hydrated dimyristoyl lecithin.1979 · 690 citations
  3. 3Free calcium ions in neurones of Helix aspersa measured with ion‐selective micro‐electrodes.1981 · 141 citations
  4. 4Effects of tetracaine on charge movements and calcium signals in frog skeletal muscle fibers1983 · 74 citations