What question did this study set out to answer?

The aim is to investigate the localization of angiotensin converting enzyme in large blood vessels, particularly in the adventitia.

July 1, 1992Journal of Hypertension

Presence of angiotensin converting enzyme in the adventitia of large blood vessels

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Population

Large blood vessels from mammals

Design

Preclinical

Authors

FRFraser M. RogersonRoyal Children's Hospital SCSiew Yeen ChaiSarawak General Hospital ISIngrid SchlaweThe University of Melbourne

Discussion

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Implication

Animal data localize adventitial ACE to vasa vasorum; leaves open relevance to human vascular disease or ACE inhibitor effects.

Key Points

The aim is to investigate the localization of angiotensin converting enzyme in large blood vessels, particularly in the adventitia.
Localized ACE in large blood vessels using in vitro autoradiography with 125I-351A.
Performed immunohistochemistry on rabbit aorta with polyclonal antisera against rabbit lung ACE.
High levels of radioligand binding observed in endothelial cells of all studied blood vessels.
Punctate binding seen in the adventitia, with immunoreactivity confined to the endothelium of the intima and vasa vasorum.
ACE inhibitors lisinopril and perindoprilat showed high affinities for binding in both endothelium and adventitia.

Structured PICO

Population

Large blood vessels from mammals (rabbit pulmonary artery, rabbit, dog and sheep aorta, human internal mammary artery and human saphenous vein)

Intervention

In vitro autoradiography using radioligand 125I-351A and immunohistochemistry

Outcome

Localization of angiotensin converting enzyme (ACE)surrogate

This study demonstrates that ACE in the adventitia of large blood vessels is localized to the vasa vasorum, providing a source of ACE distant from the luminal endothelial surface.

Cite This Study

Rogerson et al. (1992) studied this question.

synapsesocial.com/papers/6a722cb2b27f15817827f647 https://doi.org/10.1097/00004872-199207000-00003

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Presence of angiotensin converting enzyme in the adventitia of large blood vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Presence of angiotensin converting enzyme in the adventitia of large blood vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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