What question did this study set out to answer?

To identify the intracellular messengers and signal transduction mechanisms coupled to angiotensin II receptors in rat neuronal cultures.

January 1, 1991AJP Cell Physiology

Angiotensin II decreases cGMP levels in neuronal cultures from rat brain

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Population

Cultured neuronal and astrocyte glial cells prepared from the hypothalamus and brain stem of 1-day-old rats

Comparison

Angiotensin II (ANG II) vs Control and astrocyte glial cultures; also…

Design

Preclinical

Authors

CSColin SumnersPreventive Cardiology LMLinda M. MyersUniversity of Florida

Discussion

Member takes

Implication

Does not inform clinical care; leaves open in vivo relevance of neuronal cGMP modulation.

Key Points

To identify the intracellular messengers and signal transduction mechanisms coupled to angiotensin II receptors in rat neuronal cultures.
Measured cellular cGMP levels and guanylate cyclase activity in cultured neurons and astrocyte glial cells isolated from the hypothalamus and brain stem of 1-day-old rats.
Tested the inhibitory effects of the receptor antagonist [Sar1,Ile8]ANG II, calcium channel blockers (cadmium, nifedipine), and the phosphodiesterase inhibitor IBMX on ANG II action.
ANG II elicited a concentration- and time-dependent decrease in cGMP content in neuronal cultures, whereas cGMP levels in astrocyte glial cultures remained unaffected.
The ANG II-mediated cGMP reduction was abolished by [Sar1,Ile8]ANG II, cadmium, nifedipine, and IBMX, with no direct changes observed in particulate or soluble guanylate cyclase activity or cGMP efflux.

Structured PICO

Population

Cultured neuronal and astrocyte glial cells prepared from the hypothalamus and brain stem of 1-day-old rats

Intervention

Angiotensin II (ANG II)

Comparator

Control (untreated cells) and astrocyte glial cultures; also compared with co-administration of ANG II-receptor antagonist [Sar1,Ile8]ANG II, calcium channel blockers (cadmium, nifedipine), or phosphodiesterase inhibitor (3-isobutyl-1-methylxanthine)

Outcome

Cellular guanosine 3',5'-cyclic monophosphate (cGMP) levelssurrogate

Angiotensin II decreases cGMP levels in cultured rat neurons via a mechanism involving calcium entry and phosphodiesterase activation, demonstrating a receptor-mediated effect on an intracellular messenger.

Cite This Study

Sumners et al. (1991) studied this question.

synapsesocial.com/papers/6a72bbfbe36a167817e40a9e https://doi.org/10.1152/ajpcell.1991.260.1.c79

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Angiotensin II decreases cGMP levels in neuronal cultures from rat brain

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Angiotensin II decreases cGMP levels in neuronal cultures from rat brain

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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