July 1, 1998Clinical and Diagnostic Laboratory ImmunologyOpen Access

Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays

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Population

128 sera from patients with suspected scrub typhus from Korat, Thailand, and 74 serum specimens from healthy…

Comparison

Enzyme-linked immunosorbent assay using refolded… vs ELISA using purified whole-cell lysate of the…

Design

Other

Authors

WCWei‐Mei ChingNaval Medical Research Command HWH. WangUniversity of Alberta CEChirapa EamsilaArmed Forces Research Institute of Medical Science

Discussion

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Implication

r56 ELISA may enable standardized scrub typhus serology; leaves open prospective validation before clinical adoption.

Structured PICO

Population

128 sera from patients with suspected scrub typhus from Korat, Thailand, and 74 serum specimens from healthy Thai soldiers, along with rabbit antisera.

Intervention

Enzyme-linked immunosorbent assay (ELISA) using refolded truncated recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi.

Comparator

ELISA using purified whole-cell lysate of the Karp strain of O. tsutsugamushi, and indirect immunoperoxidase assay as the reference standard.

Outcome

Sensitivity and specificity for detection of IgG and IgM antibodies.surrogate

Purified recombinant r56 antigen demonstrates high sensitivity and specificity in ELISA, suggesting it is a suitable replacement for whole-cell antigens in scrub typhus diagnostic assays.

Cite This Study

Ching et al. (1998) studied this question.

synapsesocial.com/papers/6a7424c8ca963d7f80391ccc https://doi.org/10.1128/cdli.5.4.519-526.1998

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Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider