What question did this study set out to answer?

To investigate the cardiovascular effects of endothelin and assess its physiological interaction with endothelium-derived relaxing factor in coronary circulation.

January 1, 1990Cardiovascular Research

Functional state of the endothelium determines the response to endothelin in the coronary circulation

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Authors

DSDuncan J. Stewart RBRichard Baffour

Discussion

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Overview

Laboratory study demonstrates that endothelial disruption unmasks endothelin-induced coronary vasoconstriction in isolated rabbit hearts, highlighting the protective role of the endothelial barrier.

Key Points

To investigate the cardiovascular effects of endothelin and assess its physiological interaction with endothelium-derived relaxing factor in coronary circulation.
Perfused isolated hearts from 15 New Zealand white rabbits using the Langendorff technique at a constant coronary flow rate of 35 ml·min⁻¹.
Administered bolus endothelin injections (1–300 ng) alone and following treatment with indomethacin (10 µM), haemoglobin (10 µM), or air embolisation (0.5 ml).
Endothelin alone caused no consistent increase in perfusion pressure, and indomethacin (10 µM) did not alter vascular responses.
Endothelium-dependent dilation inhibition via haemoglobin unmasked a dose-dependent constriction with an EC50 of 41 (SEM 15) ng and a maximum pressure increase of +46 (SEM 8) mm Hg.
Air embolisation further augmented vasoconstriction (EC50 26 [SEM 10] ng, maximum +102 [SEM 12] mm Hg) and induced a marked reduction in left ventricular contractile function.

Cite This Study

Stewart et al. (1990) studied this question.

synapsesocial.com/papers/6a7428d03044697dfb6e873f https://doi.org/10.1093/cvr/24.1.7

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January 1, 1990Cardiovascular Research

Functional state of the endothelium determines the response to endothelin in the coronary circulation

View Full Paper

Ask AI

Bookmark

Authors

DSDuncan J. Stewart RBRichard Baffour

Discussion

Member takes

Overview

Laboratory study demonstrates that endothelial disruption unmasks endothelin-induced coronary vasoconstriction in isolated rabbit hearts, highlighting the protective role of the endothelial barrier.

Key Points

To investigate the cardiovascular effects of endothelin and assess its physiological interaction with endothelium-derived relaxing factor in coronary circulation.
Perfused isolated hearts from 15 New Zealand white rabbits using the Langendorff technique at a constant coronary flow rate of 35 ml·min⁻¹.
Administered bolus endothelin injections (1–300 ng) alone and following treatment with indomethacin (10 µM), haemoglobin (10 µM), or air embolisation (0.5 ml).
Endothelin alone caused no consistent increase in perfusion pressure, and indomethacin (10 µM) did not alter vascular responses.
Endothelium-dependent dilation inhibition via haemoglobin unmasked a dose-dependent constriction with an EC50 of 41 (SEM 15) ng and a maximum pressure increase of +46 (SEM 8) mm Hg.
Air embolisation further augmented vasoconstriction (EC50 26 [SEM 10] ng, maximum +102 [SEM 12] mm Hg) and induced a marked reduction in left ventricular contractile function.

Cite This Study

Stewart et al. (1990) studied this question.

synapsesocial.com/papers/6a7428d03044697dfb6e873f https://doi.org/10.1093/cvr/24.1.7

View Full Paper

Ask AI

Bookmark