Fasting recovers age-related hypertension in the rats: reset of renal renin-angiotensin system components and klotho

Fig. 1. (A) Systolic blood pressure (SBP), *P < 0.05 and ***P < 0.001 versus regimen-matched group. ###P < 0.001 vs. age-matched AL group. (B) diastolic blood pressure (DBP), *P < 0.05 and ***P < 0.001 versus regimen-matched group. #P < 0.05 vs. age-matched AL group and ###P < 0.001 vs. age-matched AL, FW group. (C) the mean arterial pressure (MAP) in different animal groups. * P < 0. 05 vs. regimen-matched young group, ***P < 0.001 vs. regimen-matched young and middle-aged group. ###P < 0.001 vs. age-matched AL group. (D) The plasma levels of Ang II in the rats. Values were measured using a rat Elisa kit. Data are represented as mean ± SEM, analysis was performed by one way ANOVA, 6 animals per group. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. regimen-matched young group. ##P < 0.01 and ###P < 0.001 vs. age-matched AL group. The measurements were from AL, ad libitum regimen; FW, fed ad libitum and fasted 1 day per week; EOD, fed ad libitum and fasted every other day

Fig. 2. The levels of (A) AT1aR mRNA and (B) AT1aR protein expression in the kidney of rats. The samples were from young, middle-aged and old animals. AT1aR mRNA levels in the kidney were measured by real-time PCR. AT1aR protein levels were measured by Western blot technique. Data are represented as mean ± SEM; analysis was performed by one-way ANOVA, 6 animals per group

Fig. 3. The levels of (A) AT2R mRNA and (B) AT2R protein expression in the kidney of rats. The samples were from young, middle-aged and old animals. AT2R mRNA levels in the kidney were measured by real-time PCR. AT2R protein levels were measured by Western blot technique. Data are represented as mean ± SEM; analysis was performed by one-way ANOVA, 6 animals per group. * P < 0.05 vs. age-matched FW group, **P < 0.01 and ***P < 0.001 vs. age-matched AL group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. regimen-matched young group

Fig. 4. The levels of (A) ACE2 mRNA and (B) ACE2 protein expression in the kidney of rats. The samples were from young, middle-aged, and old animals. ACE2 mRNA levels in the kidney were measured by real-time PCR. ACE2 protein levels were measured by Western blot technique. Data are represented as mean ± SEM; analysis was performed by one-way ANOVA, 6 animals per group. * P < 0.05 and ***P < 0.001 vs. age-matched AL group. **P < 0.01 vs. regimen-matched middle-aged group. #P < 0.05 and ###P < 0.001 vs. regimen-matched young group. ACE2, angiotensin-converting enzyme 2

Fig. 5. (A)The ratio of angiotensin II receptor 1a (AT1aR)/angiotensin II receptor 2 (AT2R) proteins in the kidney of rats. **P < 0.01 vs. age-matched AL, FW group. # P < 0.05 vs. regimen-matched young group. (B) The plasma levels of α- Klotho in different groups. *P < 0.05 and ***P < 0.001 vs. regimen-matched young and middle -aged group. ###P < 0.001 vs. age-matched AL, FW group. The samples were from young, middle-aged and old animals. AL, ad libitum regimen; FW, fed ad libitum and fasted 1 day per week; EOD, fed ad libitum and fasted every other day. Data are represented as mean ± SEM; analysis was performed by one-way ANOVA, 6 animals per group