Key result
Embryonic stem cell-derived cardiomyocytes exhibit immature morphology, sinus nodal-like electrophysiology, and higher dependence on trans-sarcolemmal calcium influx compared to adult cardiomyocytes.
This review highlights the developmental differences in electrophysiology and calcium handling between embryonic stem cell-derived cardiomyocytes and adult cardiomyocytes, which is important for their use in cardiac repair and pharmacological testing.
Maturity deficits in ESC-derived cardiomyocytes caution against direct clinical translation; leaves open refinement of stem cell protocols.
Embryonic stem cell-derived cardiomyocytes (ESC-CMs) hold great interest in many fields of research including clinical applications such as stem cell and gene therapy for cardiac repair or regeneration. ESC-CMs are also used as a platform tool for pharmacological tests or for investigations of cardiac remodeling. ESC-CMs have many different aspects of morphology, electrophysiology, calcium handling, and bioenergetics compared with adult cardiomyocytes. They are immature in morphology, similar to sinus nodal-like in the electrophysiology, higher contribution of trans-sarcolemmal Ca 2+ influx to Ca 2+ handling, and higher dependence on anaerobic glycolysis. Here, I review a detailed electrophysiology and Ca 2+ handling features of ESC-CMs during differentiation into adult cardiomyocytes to gain insights into how all the developmental changes are related to each other to display cardinal features of developing cardiomyocytes.
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Jae Boum Youm (2016) reported a review. Embryonic stem cell-derived cardiomyocytes vs. Adult cardiomyocytes was evaluated. Embryonic stem cell-derived cardiomyocytes exhibit immature morphology, sinus nodal-like electrophysiology, and higher dependence on trans-sarcolemmal calcium influx compared to adult cardiomyocytes.
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